The possible mechanism of rhapontigenin influencing antifungal activity on Candida albicans

Narae Kim, Jeong Keun Kim, Dahyun Hwang, Young Hee Lim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Rhapontigenin, an aglycone of rhapontin, was produced by biotransformation and we investigated its antifungal activity against Candida albicans, one of the most important opportunistic fungal pathogens. Rhapontigenin is found to have, in vitro, inhibitory activity with a minimal inhibitory concentration (MIC) value against all test isolates of 128 - 256 μg/ml. We detected increased reactive oxygen species (ROS) levels in yeast cultures treated with rhapontigenin at the MIC. Rhapontigenin inhibited DNA, RNA, and protein synthesis, especially RNA synthesis, and induced morphological changes and apoptosis of C. albicans. The apoptotic effect of rhapontigenin on C. albicans at subinhibitory concentrations was higher in the stationary growth phase than in the exponential phase, while the opposite results were noted with amphotericin B. The mechanism of antifungal activity of rhapontigenin may be associated with the generation of ROS that might induce apoptosis and it may also involve the inhibition of ergosterol biosynthesis.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalMedical Mycology
Volume51
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

Fingerprint

Candida albicans
reactive oxygen species
apoptosis
RNA
amphotericin B
ergosterol
biotransformation
Reactive Oxygen Species
protein synthesis
biosynthesis
Apoptosis
yeasts
Ergosterol
synthesis
pathogens
Amphotericin B
DNA
Biotransformation
rhapontigenin
Yeasts

Keywords

  • Antifungal activity
  • Apoptosis
  • Candida albicans
  • Ergosterol
  • Rhapontigenin

ASJC Scopus subject areas

  • veterinary(all)
  • Infectious Diseases

Cite this

The possible mechanism of rhapontigenin influencing antifungal activity on Candida albicans. / Kim, Narae; Kim, Jeong Keun; Hwang, Dahyun; Lim, Young Hee.

In: Medical Mycology, Vol. 51, No. 1, 01.01.2013, p. 45-52.

Research output: Contribution to journalArticle

Kim, Narae ; Kim, Jeong Keun ; Hwang, Dahyun ; Lim, Young Hee. / The possible mechanism of rhapontigenin influencing antifungal activity on Candida albicans. In: Medical Mycology. 2013 ; Vol. 51, No. 1. pp. 45-52.
@article{beee4d4c8e5d4a7c9e2ce8bf15704cac,
title = "The possible mechanism of rhapontigenin influencing antifungal activity on Candida albicans",
abstract = "Rhapontigenin, an aglycone of rhapontin, was produced by biotransformation and we investigated its antifungal activity against Candida albicans, one of the most important opportunistic fungal pathogens. Rhapontigenin is found to have, in vitro, inhibitory activity with a minimal inhibitory concentration (MIC) value against all test isolates of 128 - 256 μg/ml. We detected increased reactive oxygen species (ROS) levels in yeast cultures treated with rhapontigenin at the MIC. Rhapontigenin inhibited DNA, RNA, and protein synthesis, especially RNA synthesis, and induced morphological changes and apoptosis of C. albicans. The apoptotic effect of rhapontigenin on C. albicans at subinhibitory concentrations was higher in the stationary growth phase than in the exponential phase, while the opposite results were noted with amphotericin B. The mechanism of antifungal activity of rhapontigenin may be associated with the generation of ROS that might induce apoptosis and it may also involve the inhibition of ergosterol biosynthesis.",
keywords = "Antifungal activity, Apoptosis, Candida albicans, Ergosterol, Rhapontigenin",
author = "Narae Kim and Kim, {Jeong Keun} and Dahyun Hwang and Lim, {Young Hee}",
year = "2013",
month = "1",
day = "1",
doi = "10.3109/13693786.2012.689021",
language = "English",
volume = "51",
pages = "45--52",
journal = "Medical Mycology",
issn = "1369-3786",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - The possible mechanism of rhapontigenin influencing antifungal activity on Candida albicans

AU - Kim, Narae

AU - Kim, Jeong Keun

AU - Hwang, Dahyun

AU - Lim, Young Hee

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Rhapontigenin, an aglycone of rhapontin, was produced by biotransformation and we investigated its antifungal activity against Candida albicans, one of the most important opportunistic fungal pathogens. Rhapontigenin is found to have, in vitro, inhibitory activity with a minimal inhibitory concentration (MIC) value against all test isolates of 128 - 256 μg/ml. We detected increased reactive oxygen species (ROS) levels in yeast cultures treated with rhapontigenin at the MIC. Rhapontigenin inhibited DNA, RNA, and protein synthesis, especially RNA synthesis, and induced morphological changes and apoptosis of C. albicans. The apoptotic effect of rhapontigenin on C. albicans at subinhibitory concentrations was higher in the stationary growth phase than in the exponential phase, while the opposite results were noted with amphotericin B. The mechanism of antifungal activity of rhapontigenin may be associated with the generation of ROS that might induce apoptosis and it may also involve the inhibition of ergosterol biosynthesis.

AB - Rhapontigenin, an aglycone of rhapontin, was produced by biotransformation and we investigated its antifungal activity against Candida albicans, one of the most important opportunistic fungal pathogens. Rhapontigenin is found to have, in vitro, inhibitory activity with a minimal inhibitory concentration (MIC) value against all test isolates of 128 - 256 μg/ml. We detected increased reactive oxygen species (ROS) levels in yeast cultures treated with rhapontigenin at the MIC. Rhapontigenin inhibited DNA, RNA, and protein synthesis, especially RNA synthesis, and induced morphological changes and apoptosis of C. albicans. The apoptotic effect of rhapontigenin on C. albicans at subinhibitory concentrations was higher in the stationary growth phase than in the exponential phase, while the opposite results were noted with amphotericin B. The mechanism of antifungal activity of rhapontigenin may be associated with the generation of ROS that might induce apoptosis and it may also involve the inhibition of ergosterol biosynthesis.

KW - Antifungal activity

KW - Apoptosis

KW - Candida albicans

KW - Ergosterol

KW - Rhapontigenin

UR - http://www.scopus.com/inward/record.url?scp=84870955517&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870955517&partnerID=8YFLogxK

U2 - 10.3109/13693786.2012.689021

DO - 10.3109/13693786.2012.689021

M3 - Article

VL - 51

SP - 45

EP - 52

JO - Medical Mycology

JF - Medical Mycology

SN - 1369-3786

IS - 1

ER -