The presence of CD8+ invariant NKT cells in mice

Hyunji Lee, Changwan Hong, Junghoon Shin, Soohwan Oh, Sundo Jung, Yoon Kyung Park, Seokmann Hong, Gap Ryol Lee, Se-Ho Park

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Invariant natural killer T (iNKT) cells develop in the thymus upon recognition of CD1d expressed on developing thymocytes. Although CD4 and CD8 coreceptors are not directly involved in the interaction between CD1d and the T cell receptors (TCRs) of iNKT cells, a conspicuous lack of CD8+ iNKT cells in mice raised the question of whether CD8+ iNKT cells are excluded due to negative selection during their thymic development, or if there is no lineage commitment for the development of murine CD8+ iNKT cells. To address this question, we analyzed iNKT cell-specific TCR Vα14+ transgenic mice, where the Vα14 transgene forces the generation of iNKT cells. This allows detailed study of the iNKT cell repertoire. We were able to identify CD8+ iNKT cells which respond to the NKT cell-specific glycolipid ligand α-galactosylceramide. Unlike conventional iNKT cells, CD8+ iNKT cells produce predominantly IFN-γ but not IL-4 upon antigen stimulation. We also confirmed the presence of CD8 + iNKT cells in wild type mice. Our results suggest that CD8 + NKT cells do exist in mice, although their population size is quite small. Their Th1-skewed phenotype might explain why the population size of this subtype needs to be controlled tightly.

Original languageEnglish
Pages (from-to)866-872
Number of pages7
JournalExperimental and Molecular Medicine
Volume41
Issue number12
DOIs
Publication statusPublished - 2009 Dec 31

Fingerprint

Natural Killer T-Cells
T-cells
T-Cell Antigen Receptor
Population Density
Galactosylceramides
Thymus
Glycolipids
Interleukin-4
Thymocytes
Transgenes
Thymus Gland
Transgenic Mice

Keywords

  • α-galactosylceramide
  • Antigens, CD1d
  • CD8-positive T-lymphocytes
  • Mice, transgenic
  • Natural killer T-cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Molecular Medicine
  • Clinical Biochemistry

Cite this

The presence of CD8+ invariant NKT cells in mice. / Lee, Hyunji; Hong, Changwan; Shin, Junghoon; Oh, Soohwan; Jung, Sundo; Park, Yoon Kyung; Hong, Seokmann; Lee, Gap Ryol; Park, Se-Ho.

In: Experimental and Molecular Medicine, Vol. 41, No. 12, 31.12.2009, p. 866-872.

Research output: Contribution to journalArticle

Lee, H, Hong, C, Shin, J, Oh, S, Jung, S, Park, YK, Hong, S, Lee, GR & Park, S-H 2009, 'The presence of CD8+ invariant NKT cells in mice', Experimental and Molecular Medicine, vol. 41, no. 12, pp. 866-872. https://doi.org/10.3858/emm.2009.41.12.092
Lee H, Hong C, Shin J, Oh S, Jung S, Park YK et al. The presence of CD8+ invariant NKT cells in mice. Experimental and Molecular Medicine. 2009 Dec 31;41(12):866-872. https://doi.org/10.3858/emm.2009.41.12.092
Lee, Hyunji ; Hong, Changwan ; Shin, Junghoon ; Oh, Soohwan ; Jung, Sundo ; Park, Yoon Kyung ; Hong, Seokmann ; Lee, Gap Ryol ; Park, Se-Ho. / The presence of CD8+ invariant NKT cells in mice. In: Experimental and Molecular Medicine. 2009 ; Vol. 41, No. 12. pp. 866-872.
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