The Probability of Neurotransmitter Release Governs AMPA Receptor Trafficking via Activity-Dependent Regulation of mGluR1 Surface Expression

Thomas M. Sanderson, Clarrisa A. Bradley, John Georgiou, Yun Hwa Hong, Ai Na Ng, Yeseul Lee, Hee Dae Kim, Doyeon Kim, Mascia Amici, Gi Hoon Son, Min Zhuo, Kyungjin Kim, Bong Kiun Kaang, Sang Jeong Kim, Graham L. Collingridge

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A major mechanism contributing to synaptic plasticity involves alterations in the number of AMPA receptors (AMPARs) expressed at synapses. Hippocampal CA1 synapses, where this process has been most extensively studied, are highly heterogeneous with respect to their probability of neurotransmitter release, P(r). It is unknown whether there is any relationship between the extent of plasticity-related AMPAR trafficking and the initial P(r) of a synapse. To address this question, we induced metabotropic glutamate receptor (mGluR) dependent long-term depression (mGluR-LTD) and assessed AMPAR trafficking and P(r) at individual synapses, using SEP-GluA2 and FM4-64, respectively. We found that either pharmacological or synaptic activation of mGluR1 reduced synaptic SEP-GluA2 in a manner that depends upon P(r); this process involved an activity-dependent reduction in surface mGluR1 that selectively protects high-P(r) synapses from synaptic weakening. Consequently, the extent of postsynaptic plasticity can be pre-tuned by presynaptic activity.

Original languageEnglish
Pages (from-to)3631-3646.e3
JournalCell Reports
Volume25
Issue number13
DOIs
Publication statusPublished - 2018 Dec 26

Fingerprint

AMPA Receptors
Synapses
Plasticity
Neurotransmitter Agents
Metabotropic Glutamate Receptors
Chemical activation
Neuronal Plasticity
metabotropic glutamate receptor type 1
Pharmacology

Keywords

  • AMPA
  • DHPG
  • FM dye
  • Long-term depression
  • LTD
  • metabotropic
  • mGluR
  • P(r)
  • probability of release
  • theta burst

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Sanderson, T. M., Bradley, C. A., Georgiou, J., Hong, Y. H., Ng, A. N., Lee, Y., ... Collingridge, G. L. (2018). The Probability of Neurotransmitter Release Governs AMPA Receptor Trafficking via Activity-Dependent Regulation of mGluR1 Surface Expression. Cell Reports, 25(13), 3631-3646.e3. https://doi.org/10.1016/j.celrep.2018.12.010

The Probability of Neurotransmitter Release Governs AMPA Receptor Trafficking via Activity-Dependent Regulation of mGluR1 Surface Expression. / Sanderson, Thomas M.; Bradley, Clarrisa A.; Georgiou, John; Hong, Yun Hwa; Ng, Ai Na; Lee, Yeseul; Kim, Hee Dae; Kim, Doyeon; Amici, Mascia; Son, Gi Hoon; Zhuo, Min; Kim, Kyungjin; Kaang, Bong Kiun; Kim, Sang Jeong; Collingridge, Graham L.

In: Cell Reports, Vol. 25, No. 13, 26.12.2018, p. 3631-3646.e3.

Research output: Contribution to journalArticle

Sanderson, TM, Bradley, CA, Georgiou, J, Hong, YH, Ng, AN, Lee, Y, Kim, HD, Kim, D, Amici, M, Son, GH, Zhuo, M, Kim, K, Kaang, BK, Kim, SJ & Collingridge, GL 2018, 'The Probability of Neurotransmitter Release Governs AMPA Receptor Trafficking via Activity-Dependent Regulation of mGluR1 Surface Expression', Cell Reports, vol. 25, no. 13, pp. 3631-3646.e3. https://doi.org/10.1016/j.celrep.2018.12.010
Sanderson, Thomas M. ; Bradley, Clarrisa A. ; Georgiou, John ; Hong, Yun Hwa ; Ng, Ai Na ; Lee, Yeseul ; Kim, Hee Dae ; Kim, Doyeon ; Amici, Mascia ; Son, Gi Hoon ; Zhuo, Min ; Kim, Kyungjin ; Kaang, Bong Kiun ; Kim, Sang Jeong ; Collingridge, Graham L. / The Probability of Neurotransmitter Release Governs AMPA Receptor Trafficking via Activity-Dependent Regulation of mGluR1 Surface Expression. In: Cell Reports. 2018 ; Vol. 25, No. 13. pp. 3631-3646.e3.
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