The protective role of uteroglobin through the modulation of tissue transglutaminase in the experimental crescentic glomerulonephritis

Seung Hee Yang, Sung Joon Shin, Ji Eun Oh, Ji Zhe Jin, Namhyun Chung, Chun Soo Lim, Suhnggwon Kim, Yon Su Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background and methods. Tissue transglutaminase (tTG) may induce pro-inflammatory cytokines and produce irreversible end-products, thus promoting renal scarring. It has recently been confirmed that the crescent formation in murine experimental crescentic glomerulonephritis (ecGN) has been inhibited by the administration of recombinant uteroglobin (rUG). However, the ability of UG on tTG modulation has not been thoroughly assessed. In this study, we investigated the feasible protective role of UG in murine ecGN through the modulation of tTG and TGF-β1 expressions. ecGN was induced by the administration of anti-GBM Ab into C57BL/6 mice. Results. Both proteinuria and BUN levels were distinctively lower in rUG-treated mice compared to those of disease control mice. Glomerular injuries such as mesangial proliferation, matrix production and crescent formation were lessened with the rUG treatment, and these findings were parallel with the attenuated expression of tTG and TGF-β1. tTG and TGF-β1 were expressed mainly on mesangial areas by the induction of ecGN and rUG treatment markedly attenuated the expressions of these proteins in glomeruli without spatial changes. With the addition of LPS to mesangial cells, the expressions of tTG and TGF-β1 were up-regulated, whilst the addition of cysteamine, tTG inhibitor, attenuated the expression of tTG and TGF-β1 as well as the cellular proliferation which was further induced by LPS. Conclusion. We demonstrate for the first time that rUG is able to attenuate the renal injury through the modulation of expressions of tTG and TGF-β1 in ecGN and further suggest a wide range of feasible molecular targets to reduce the severity of human glomerulonephritis.

Original languageEnglish
Pages (from-to)3437-3445
Number of pages9
JournalNephrology Dialysis Transplantation
Volume23
Issue number11
DOIs
Publication statusPublished - 2008 Nov 1

Fingerprint

Uteroglobin
Glomerulonephritis
Kidney
Cysteamine
transglutaminase 2
Mesangial Cells
Blood Urea Nitrogen
Wounds and Injuries
Inbred C57BL Mouse
Proteinuria
Cicatrix
transglutaminase 1
Cell Proliferation
Cytokines

Keywords

  • Experimental crescentic glomerulonephritis
  • Tissue transglutaminase
  • Uteroglobin

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

The protective role of uteroglobin through the modulation of tissue transglutaminase in the experimental crescentic glomerulonephritis. / Yang, Seung Hee; Shin, Sung Joon; Oh, Ji Eun; Jin, Ji Zhe; Chung, Namhyun; Lim, Chun Soo; Kim, Suhnggwon; Kim, Yon Su.

In: Nephrology Dialysis Transplantation, Vol. 23, No. 11, 01.11.2008, p. 3437-3445.

Research output: Contribution to journalArticle

Yang, Seung Hee ; Shin, Sung Joon ; Oh, Ji Eun ; Jin, Ji Zhe ; Chung, Namhyun ; Lim, Chun Soo ; Kim, Suhnggwon ; Kim, Yon Su. / The protective role of uteroglobin through the modulation of tissue transglutaminase in the experimental crescentic glomerulonephritis. In: Nephrology Dialysis Transplantation. 2008 ; Vol. 23, No. 11. pp. 3437-3445.
@article{e837f554f9e241a68a9efa6dc07c2002,
title = "The protective role of uteroglobin through the modulation of tissue transglutaminase in the experimental crescentic glomerulonephritis",
abstract = "Background and methods. Tissue transglutaminase (tTG) may induce pro-inflammatory cytokines and produce irreversible end-products, thus promoting renal scarring. It has recently been confirmed that the crescent formation in murine experimental crescentic glomerulonephritis (ecGN) has been inhibited by the administration of recombinant uteroglobin (rUG). However, the ability of UG on tTG modulation has not been thoroughly assessed. In this study, we investigated the feasible protective role of UG in murine ecGN through the modulation of tTG and TGF-β1 expressions. ecGN was induced by the administration of anti-GBM Ab into C57BL/6 mice. Results. Both proteinuria and BUN levels were distinctively lower in rUG-treated mice compared to those of disease control mice. Glomerular injuries such as mesangial proliferation, matrix production and crescent formation were lessened with the rUG treatment, and these findings were parallel with the attenuated expression of tTG and TGF-β1. tTG and TGF-β1 were expressed mainly on mesangial areas by the induction of ecGN and rUG treatment markedly attenuated the expressions of these proteins in glomeruli without spatial changes. With the addition of LPS to mesangial cells, the expressions of tTG and TGF-β1 were up-regulated, whilst the addition of cysteamine, tTG inhibitor, attenuated the expression of tTG and TGF-β1 as well as the cellular proliferation which was further induced by LPS. Conclusion. We demonstrate for the first time that rUG is able to attenuate the renal injury through the modulation of expressions of tTG and TGF-β1 in ecGN and further suggest a wide range of feasible molecular targets to reduce the severity of human glomerulonephritis.",
keywords = "Experimental crescentic glomerulonephritis, Tissue transglutaminase, Uteroglobin",
author = "Yang, {Seung Hee} and Shin, {Sung Joon} and Oh, {Ji Eun} and Jin, {Ji Zhe} and Namhyun Chung and Lim, {Chun Soo} and Suhnggwon Kim and Kim, {Yon Su}",
year = "2008",
month = "11",
day = "1",
doi = "10.1093/ndt/gfn268",
language = "English",
volume = "23",
pages = "3437--3445",
journal = "Nephrology Dialysis Transplantation",
issn = "0931-0509",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - The protective role of uteroglobin through the modulation of tissue transglutaminase in the experimental crescentic glomerulonephritis

AU - Yang, Seung Hee

AU - Shin, Sung Joon

AU - Oh, Ji Eun

AU - Jin, Ji Zhe

AU - Chung, Namhyun

AU - Lim, Chun Soo

AU - Kim, Suhnggwon

AU - Kim, Yon Su

PY - 2008/11/1

Y1 - 2008/11/1

N2 - Background and methods. Tissue transglutaminase (tTG) may induce pro-inflammatory cytokines and produce irreversible end-products, thus promoting renal scarring. It has recently been confirmed that the crescent formation in murine experimental crescentic glomerulonephritis (ecGN) has been inhibited by the administration of recombinant uteroglobin (rUG). However, the ability of UG on tTG modulation has not been thoroughly assessed. In this study, we investigated the feasible protective role of UG in murine ecGN through the modulation of tTG and TGF-β1 expressions. ecGN was induced by the administration of anti-GBM Ab into C57BL/6 mice. Results. Both proteinuria and BUN levels were distinctively lower in rUG-treated mice compared to those of disease control mice. Glomerular injuries such as mesangial proliferation, matrix production and crescent formation were lessened with the rUG treatment, and these findings were parallel with the attenuated expression of tTG and TGF-β1. tTG and TGF-β1 were expressed mainly on mesangial areas by the induction of ecGN and rUG treatment markedly attenuated the expressions of these proteins in glomeruli without spatial changes. With the addition of LPS to mesangial cells, the expressions of tTG and TGF-β1 were up-regulated, whilst the addition of cysteamine, tTG inhibitor, attenuated the expression of tTG and TGF-β1 as well as the cellular proliferation which was further induced by LPS. Conclusion. We demonstrate for the first time that rUG is able to attenuate the renal injury through the modulation of expressions of tTG and TGF-β1 in ecGN and further suggest a wide range of feasible molecular targets to reduce the severity of human glomerulonephritis.

AB - Background and methods. Tissue transglutaminase (tTG) may induce pro-inflammatory cytokines and produce irreversible end-products, thus promoting renal scarring. It has recently been confirmed that the crescent formation in murine experimental crescentic glomerulonephritis (ecGN) has been inhibited by the administration of recombinant uteroglobin (rUG). However, the ability of UG on tTG modulation has not been thoroughly assessed. In this study, we investigated the feasible protective role of UG in murine ecGN through the modulation of tTG and TGF-β1 expressions. ecGN was induced by the administration of anti-GBM Ab into C57BL/6 mice. Results. Both proteinuria and BUN levels were distinctively lower in rUG-treated mice compared to those of disease control mice. Glomerular injuries such as mesangial proliferation, matrix production and crescent formation were lessened with the rUG treatment, and these findings were parallel with the attenuated expression of tTG and TGF-β1. tTG and TGF-β1 were expressed mainly on mesangial areas by the induction of ecGN and rUG treatment markedly attenuated the expressions of these proteins in glomeruli without spatial changes. With the addition of LPS to mesangial cells, the expressions of tTG and TGF-β1 were up-regulated, whilst the addition of cysteamine, tTG inhibitor, attenuated the expression of tTG and TGF-β1 as well as the cellular proliferation which was further induced by LPS. Conclusion. We demonstrate for the first time that rUG is able to attenuate the renal injury through the modulation of expressions of tTG and TGF-β1 in ecGN and further suggest a wide range of feasible molecular targets to reduce the severity of human glomerulonephritis.

KW - Experimental crescentic glomerulonephritis

KW - Tissue transglutaminase

KW - Uteroglobin

UR - http://www.scopus.com/inward/record.url?scp=54149085961&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54149085961&partnerID=8YFLogxK

U2 - 10.1093/ndt/gfn268

DO - 10.1093/ndt/gfn268

M3 - Article

VL - 23

SP - 3437

EP - 3445

JO - Nephrology Dialysis Transplantation

JF - Nephrology Dialysis Transplantation

SN - 0931-0509

IS - 11

ER -