The proteomic analysis of an adipocyte differentiated from human mesenchymal stem cells using two-dimensional gel electrophoresis

Hyun Kyung Lee, Byung Hyo Lee, Seung Ah Park, Chan Wha Kim

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Adipose tissues play a crucial endocrine role in the control of whole body glucose homeostasis and insulin sensitivity. Considering the current substantial rise in obesity and obesity-related diseases, including diabetes, it is important to understand the molecular basis of adipocyte differentiation and its control. In this study, we have analyzed the protein expression inherent to adipogenic differentiation, by 2-DE, MALDI-TOF, and RT-PCR. This study focused on proteins that were differentially expressed by the differentiation of human mesenchymal stem cells (hMSCs) to adipocytes. We conducted 2-DE for each set of proteins in the cytosol of adipocytes that had differentiated from hMSC, in a pH range from 3-10. Thirty-two protein spots were shown to have different expression levels. Among these, eight up-regulated proteins were identified by MALDI-TOF/MS, as the following: syntaxin binding protein 3, OSBP-related protein 3, phosphodiesterase, glycophorin, immunoglobulin kappa chain variable region, peroxisome proliferative activated receptor gamma (PPAR-γ), bA528A10.3.1 (novel protein similar to KIAA01616, isoform 1), and T cell receptor V-β 4. Four proteins: syntaxin-3, OSBP-related protein 3, PPAR-γ and glycophorin were associated with adipogenesis.

Original languageEnglish
Pages (from-to)1223-1229
Number of pages7
JournalProteomics
Volume6
Issue number4
DOIs
Publication statusPublished - 2006 Feb 1

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Electrophoresis, Gel, Two-Dimensional
Stem cells
Electrophoresis
Mesenchymal Stromal Cells
Adipocytes
Proteomics
Gels
Proteins
Qa-SNARE Proteins
Glycophorin
Peroxisomes
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Obesity
Immunoglobulin kappa-Chains
Adipogenesis
Phosphoric Diester Hydrolases
Medical problems
T-Cell Antigen Receptor
Cytosol
Insulin Resistance

Keywords

  • Adipogenesis
  • Differentiation
  • Human mesenchymal stem cells
  • Protein profile

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

The proteomic analysis of an adipocyte differentiated from human mesenchymal stem cells using two-dimensional gel electrophoresis. / Lee, Hyun Kyung; Lee, Byung Hyo; Park, Seung Ah; Kim, Chan Wha.

In: Proteomics, Vol. 6, No. 4, 01.02.2006, p. 1223-1229.

Research output: Contribution to journalArticle

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