Abstract
Protease HslV and ATPase HslU form an ATP-dependent protease in bacteria. We have previously determined the structure of the components of this protease. In the case of HslU, the structure was derived from HslU-HslV cocrystals, combining phase information from MAD and the previously determined HslV model. Whereas the structures of the components were confirmed in detail by later structures, the quaternary arrangement of HslV and HslU was not reproduced in later crystal forms. In a recent communication to this journal, Wang attempted a reinterpretation of our original data to account for this difference. In response, we demonstrate that difference Pattersons, difference Fouriers, molecular replacement calculations, R factors, and omit maps all support our original analysis and prove that the suggested reinterpretation is false by these criteria. In particular, we show that our crystals are essentially untwinned and that only the originally reported quaternary arrangement of HslV and HslU particles is consistent with the experimental data. We finally demonstrate that Wang's newly introduced R tpart method to predict translational corrections for a subset of the unit cell contents is systematically flawed.
| Original language | English |
|---|---|
| Pages (from-to) | 281-293 |
| Number of pages | 13 |
| Journal | Journal of Structural Biology |
| Volume | 135 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2001 Dec 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Structural Biology
Cite this
The quaternary arrangement of HslU and HslV in a cocrystal : A response to Wang, Yale. / Bochtler, Matthias; Song, Hyun Kyu; Hartmann, Claudia; Ramachandran, Ravishankar; Huber, Robert.
In: Journal of Structural Biology, Vol. 135, No. 3, 01.12.2001, p. 281-293.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The quaternary arrangement of HslU and HslV in a cocrystal
T2 - A response to Wang, Yale
AU - Bochtler, Matthias
AU - Song, Hyun Kyu
AU - Hartmann, Claudia
AU - Ramachandran, Ravishankar
AU - Huber, Robert
PY - 2001/12/1
Y1 - 2001/12/1
N2 - Protease HslV and ATPase HslU form an ATP-dependent protease in bacteria. We have previously determined the structure of the components of this protease. In the case of HslU, the structure was derived from HslU-HslV cocrystals, combining phase information from MAD and the previously determined HslV model. Whereas the structures of the components were confirmed in detail by later structures, the quaternary arrangement of HslV and HslU was not reproduced in later crystal forms. In a recent communication to this journal, Wang attempted a reinterpretation of our original data to account for this difference. In response, we demonstrate that difference Pattersons, difference Fouriers, molecular replacement calculations, R factors, and omit maps all support our original analysis and prove that the suggested reinterpretation is false by these criteria. In particular, we show that our crystals are essentially untwinned and that only the originally reported quaternary arrangement of HslV and HslU particles is consistent with the experimental data. We finally demonstrate that Wang's newly introduced R tpart method to predict translational corrections for a subset of the unit cell contents is systematically flawed.
AB - Protease HslV and ATPase HslU form an ATP-dependent protease in bacteria. We have previously determined the structure of the components of this protease. In the case of HslU, the structure was derived from HslU-HslV cocrystals, combining phase information from MAD and the previously determined HslV model. Whereas the structures of the components were confirmed in detail by later structures, the quaternary arrangement of HslV and HslU was not reproduced in later crystal forms. In a recent communication to this journal, Wang attempted a reinterpretation of our original data to account for this difference. In response, we demonstrate that difference Pattersons, difference Fouriers, molecular replacement calculations, R factors, and omit maps all support our original analysis and prove that the suggested reinterpretation is false by these criteria. In particular, we show that our crystals are essentially untwinned and that only the originally reported quaternary arrangement of HslV and HslU particles is consistent with the experimental data. We finally demonstrate that Wang's newly introduced R tpart method to predict translational corrections for a subset of the unit cell contents is systematically flawed.
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U2 - 10.1006/jsbi.2001.4413
DO - 10.1006/jsbi.2001.4413
M3 - Article
VL - 135
SP - 281
EP - 293
JO - Journal of Structural Biology
JF - Journal of Structural Biology
SN - 1047-8477
IS - 3
ER -