The Rab GTPase RabG3b functions in autophagy and contributes to tracheary element differentiation in Arabidopsis

Soon Il Kwon, Hong Joo Cho, Jin Hee Jung, Kohki Yoshimoto, Ken Shirasu, Ohkmae Kim

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The tracheary elements (TEs) of the xylem serve as the water-conducting vessels of the plant vascular system. To achieve this, TEs undergo secondary cell wall thickening and cell death, during which the cell contents are completely removed. Cell death of TEs is a typical example of developmental programmed cell death that has been suggested to be autophagic. However, little evidence of autophagy in TE differentiation has been provided. The present study demonstrates that the small GTP binding protein RabG3b plays a role in TE differentiation through its function in autophagy. Differentiating wild type TE cells were found to undergo autophagy in an Arabidopsis culture system. Both autophagy and TE formation were significantly stimulated by overexpression of a constitutively active mutant (RabG3bCA), and were inhibited in transgenic plants overexpressing a dominant negative mutant (RabG3bDN) or RabG3b RNAi (RabG3bRNAi), a brassinosteroid insensitive mutant bri1-301, and an autophagy mutant atg5-1. Taken together, our results suggest that autophagy occurs during TE differentiation, and that RabG3b, as a component of autophagy, regulates TE differentiation.

Original languageEnglish
Pages (from-to)151-164
Number of pages14
JournalPlant Journal
Volume64
Issue number1
DOIs
Publication statusPublished - 2010 Oct 1

Fingerprint

rab GTP-Binding Proteins
tracheary elements
autophagy
Autophagy
guanosinetriphosphatase
Arabidopsis
Cell Death
mutants
Brassinosteroids
cell death
Xylem
Genetically Modified Plants
GTP-Binding Proteins
plant vascular system
Cell Wall
brassinosteroids
Blood Vessels
xylem vessels
G-proteins
xylem

Keywords

  • Arabidopsis
  • autophagic cell death
  • autophagy
  • RabG3b
  • small GTP binding protein
  • tracheary element differentiation

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

The Rab GTPase RabG3b functions in autophagy and contributes to tracheary element differentiation in Arabidopsis. / Kwon, Soon Il; Cho, Hong Joo; Jung, Jin Hee; Yoshimoto, Kohki; Shirasu, Ken; Kim, Ohkmae.

In: Plant Journal, Vol. 64, No. 1, 01.10.2010, p. 151-164.

Research output: Contribution to journalArticle

Kwon, Soon Il ; Cho, Hong Joo ; Jung, Jin Hee ; Yoshimoto, Kohki ; Shirasu, Ken ; Kim, Ohkmae. / The Rab GTPase RabG3b functions in autophagy and contributes to tracheary element differentiation in Arabidopsis. In: Plant Journal. 2010 ; Vol. 64, No. 1. pp. 151-164.
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