The rab GTPase rabG3b positively regulates autophagy and immunity-associated hypersensitive cell death in Arabidopsis

Soon Il Kwon, Hong Joo Cho, Sung Ryul Kim, Ohkmae Kim

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

A central component of the plant defense response to pathogens is the hypersensitive response (HR), a form of programmed cell death (PCD). Rapid and localized induction of HR PCD ensures that pathogen invasion is prevented. Autophagy has been implicated in the regulation of HR cell death, but the functional relationship between autophagy and HR PCD and the regulation of these processes during the plant immune response remain controversial. Here, we show that a small GTP-binding protein, RabG3b, plays a positive role in autophagy and promotes HR cell death in response to avirulent bacterial pathogens in Arabidopsis (Arabidopsis thaliana). Transgenic plants overexpressing a constitutively active RabG3b (RabG3bCA) displayed accelerated, unrestricted HR PCD within 1 d of infection, in contrast to the autophagy-defective atg5-1 mutant, which gradually developed chlorotic cell death through uninfected sites over several days. Microscopic analyses showed the accumulation of autophagic structures during HR cell death in RabG3bCA cells. Our results suggest that RabG3b contributes to HR cell death via the activation of autophagy, which plays a positive role in plant immunity-triggered HR PCD.

Original languageEnglish
Pages (from-to)1722-1736
Number of pages15
JournalPlant Physiology
Volume161
Issue number4
DOIs
Publication statusPublished - 2013 Apr 1

Fingerprint

rab GTP-Binding Proteins
autophagy
Autophagy
guanosinetriphosphatase
hypersensitive response
Arabidopsis
cell death
Immunity
Cell Death
immunity
apoptosis
Plant Immunity
pathogens
Plant Structures
Genetically Modified Plants
G-proteins
GTP-Binding Proteins
transgenic plants
Arabidopsis thaliana
immune response

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

The rab GTPase rabG3b positively regulates autophagy and immunity-associated hypersensitive cell death in Arabidopsis. / Kwon, Soon Il; Cho, Hong Joo; Kim, Sung Ryul; Kim, Ohkmae.

In: Plant Physiology, Vol. 161, No. 4, 01.04.2013, p. 1722-1736.

Research output: Contribution to journalArticle

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