Abstract
A central component of the plant defense response to pathogens is the hypersensitive response (HR), a form of programmed cell death (PCD). Rapid and localized induction of HR PCD ensures that pathogen invasion is prevented. Autophagy has been implicated in the regulation of HR cell death, but the functional relationship between autophagy and HR PCD and the regulation of these processes during the plant immune response remain controversial. Here, we show that a small GTP-binding protein, RabG3b, plays a positive role in autophagy and promotes HR cell death in response to avirulent bacterial pathogens in Arabidopsis (Arabidopsis thaliana). Transgenic plants overexpressing a constitutively active RabG3b (RabG3bCA) displayed accelerated, unrestricted HR PCD within 1 d of infection, in contrast to the autophagy-defective atg5-1 mutant, which gradually developed chlorotic cell death through uninfected sites over several days. Microscopic analyses showed the accumulation of autophagic structures during HR cell death in RabG3bCA cells. Our results suggest that RabG3b contributes to HR cell death via the activation of autophagy, which plays a positive role in plant immunity-triggered HR PCD.
| Original language | English |
|---|---|
| Pages (from-to) | 1722-1736 |
| Number of pages | 15 |
| Journal | Plant Physiology |
| Volume | 161 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2013 Apr 1 |
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ASJC Scopus subject areas
- Plant Science
- Genetics
- Physiology
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The rab GTPase rabG3b positively regulates autophagy and immunity-associated hypersensitive cell death in Arabidopsis. / Kwon, Soon Il; Cho, Hong Joo; Kim, Sung Ryul; Kim, Ohkmae.
In: Plant Physiology, Vol. 161, No. 4, 01.04.2013, p. 1722-1736.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The rab GTPase rabG3b positively regulates autophagy and immunity-associated hypersensitive cell death in Arabidopsis
AU - Kwon, Soon Il
AU - Cho, Hong Joo
AU - Kim, Sung Ryul
AU - Kim, Ohkmae
PY - 2013/4/1
Y1 - 2013/4/1
N2 - A central component of the plant defense response to pathogens is the hypersensitive response (HR), a form of programmed cell death (PCD). Rapid and localized induction of HR PCD ensures that pathogen invasion is prevented. Autophagy has been implicated in the regulation of HR cell death, but the functional relationship between autophagy and HR PCD and the regulation of these processes during the plant immune response remain controversial. Here, we show that a small GTP-binding protein, RabG3b, plays a positive role in autophagy and promotes HR cell death in response to avirulent bacterial pathogens in Arabidopsis (Arabidopsis thaliana). Transgenic plants overexpressing a constitutively active RabG3b (RabG3bCA) displayed accelerated, unrestricted HR PCD within 1 d of infection, in contrast to the autophagy-defective atg5-1 mutant, which gradually developed chlorotic cell death through uninfected sites over several days. Microscopic analyses showed the accumulation of autophagic structures during HR cell death in RabG3bCA cells. Our results suggest that RabG3b contributes to HR cell death via the activation of autophagy, which plays a positive role in plant immunity-triggered HR PCD.
AB - A central component of the plant defense response to pathogens is the hypersensitive response (HR), a form of programmed cell death (PCD). Rapid and localized induction of HR PCD ensures that pathogen invasion is prevented. Autophagy has been implicated in the regulation of HR cell death, but the functional relationship between autophagy and HR PCD and the regulation of these processes during the plant immune response remain controversial. Here, we show that a small GTP-binding protein, RabG3b, plays a positive role in autophagy and promotes HR cell death in response to avirulent bacterial pathogens in Arabidopsis (Arabidopsis thaliana). Transgenic plants overexpressing a constitutively active RabG3b (RabG3bCA) displayed accelerated, unrestricted HR PCD within 1 d of infection, in contrast to the autophagy-defective atg5-1 mutant, which gradually developed chlorotic cell death through uninfected sites over several days. Microscopic analyses showed the accumulation of autophagic structures during HR cell death in RabG3bCA cells. Our results suggest that RabG3b contributes to HR cell death via the activation of autophagy, which plays a positive role in plant immunity-triggered HR PCD.
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U2 - 10.1104/pp.112.208108
DO - 10.1104/pp.112.208108
M3 - Article
C2 - 23404918
AN - SCOPUS:84875738550
VL - 161
SP - 1722
EP - 1736
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 4
ER -