The rapid destabilization of p53 mRNA in immortal chicken embryo fibroblast cells

Hyunggee Kim, Seungkwon You, Linda K. Foster, James Farris, Douglas N. Foster

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The steady-state levels of p53 mRNA were dramatically lower in immortal chicken embryo fibroblast (CEF) cell lines compared to primary CEF cells. In the presence of cycloheximide (CHX), the steady-state levels of p53 mRNA markedly increased in immortal CEF cell lines, similar to levels found in primary cells. The de novo synthetic rates of p53 mRNA were relatively similar in primary and immortal cells grown in the presence or absence of CHX. Destabilization of p53 mRNA was observed in the nuclei of immortal, but not primary, CEF cells. The half-life of p53 mRNA in primary cells was found to be a relatively long 23 h compared to only 3 h in immortal cells. The expression of transfected p53 cDNA was inhibited in immortal cells, but restored upon CHX treatment. The 5′-region of the p53 mRNA was shown to be involved in the rapid p53 mRNA destabilization in immortal cells by expression analysis of 5′- and 3′-deleted p53 cDNAs as well as fusion mRNA constructs of N-terminal p53 and N-terminal deleted LacZ genes. Together, it is suggestive that the down-regulation of p53 mRNA in immortal CEF cells occurs through a post-transcriptional destabilizing mechanism.

Original languageEnglish
Pages (from-to)5118-5123
Number of pages6
JournalOncogene
Volume20
Issue number37
DOIs
Publication statusPublished - 2001 Aug 23
Externally publishedYes

Keywords

  • CEF
  • Chicken
  • Cycloheximide
  • Immortalization
  • MRNA destablization
  • p53 mRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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