The relationship among corneal opacity and immunohistochemical expression of heat shock protein 72/73, and c-fos after excimer laser photorefractive keratectomy

J. W. Hong, Yong Yeon Kim, Hyo Myung Kim, K. Huh, T. S. Lee

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Abstract

Purpose. To determine the etiology of the corneal opacity, the author studied on inunnunohistochemical stain for HSP 72/73, c-fos after myopic excimer laser PRK. Methods. Myopic excimer laser PRK was operated on rabbit's cornea to correct -6,-9,-15 diopter of myopia. Comea opacity was examined and immunohistochemical stain was performed for Hsp72/73 and c-fos. Results. Corneal opacity was founded at density 0.83 (-6 group), l(-9 group), 1.67(-15 group) Sweek after operation. HSP 72 was expressed in corneal epithelium at the -15 group 24 hours after operation and maximal expression was obtained at 48 hours after operation. Hsp 73 was seen at all group in corneal epithelium and stroma. c-fos was seen in corneal epithelium at ohour after operation in all group. But maximal expression was seen at 24hour after operation in -IS group. Conclusion Corneal opacity was developed at -15D group with statistical significance. Corneal epithelial hyperplasia, increased number of stromal keratocytes, hsp 72 expression and c-fos expression could be significant factors for the corneal opacity.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
Publication statusPublished - 1997 Dec 1

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Laser Corneal Surgery
HSP72 Heat-Shock Proteins
Photorefractive Keratectomy
Corneal Opacity
Excimer Lasers
Corneal Epithelium
Coloring Agents
Corneal Stroma
Cornea
Hyperplasia
Rabbits

ASJC Scopus subject areas

  • Ophthalmology

Cite this

@article{48724f4d660c49bdbedfb70fb9dfc9ff,
title = "The relationship among corneal opacity and immunohistochemical expression of heat shock protein 72/73, and c-fos after excimer laser photorefractive keratectomy",
abstract = "Purpose. To determine the etiology of the corneal opacity, the author studied on inunnunohistochemical stain for HSP 72/73, c-fos after myopic excimer laser PRK. Methods. Myopic excimer laser PRK was operated on rabbit's cornea to correct -6,-9,-15 diopter of myopia. Comea opacity was examined and immunohistochemical stain was performed for Hsp72/73 and c-fos. Results. Corneal opacity was founded at density 0.83 (-6 group), l(-9 group), 1.67(-15 group) Sweek after operation. HSP 72 was expressed in corneal epithelium at the -15 group 24 hours after operation and maximal expression was obtained at 48 hours after operation. Hsp 73 was seen at all group in corneal epithelium and stroma. c-fos was seen in corneal epithelium at ohour after operation in all group. But maximal expression was seen at 24hour after operation in -IS group. Conclusion Corneal opacity was developed at -15D group with statistical significance. Corneal epithelial hyperplasia, increased number of stromal keratocytes, hsp 72 expression and c-fos expression could be significant factors for the corneal opacity.",
author = "Hong, {J. W.} and Kim, {Yong Yeon} and Kim, {Hyo Myung} and K. Huh and Lee, {T. S.}",
year = "1997",
month = "12",
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language = "English",
volume = "38",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
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TY - JOUR

T1 - The relationship among corneal opacity and immunohistochemical expression of heat shock protein 72/73, and c-fos after excimer laser photorefractive keratectomy

AU - Hong, J. W.

AU - Kim, Yong Yeon

AU - Kim, Hyo Myung

AU - Huh, K.

AU - Lee, T. S.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Purpose. To determine the etiology of the corneal opacity, the author studied on inunnunohistochemical stain for HSP 72/73, c-fos after myopic excimer laser PRK. Methods. Myopic excimer laser PRK was operated on rabbit's cornea to correct -6,-9,-15 diopter of myopia. Comea opacity was examined and immunohistochemical stain was performed for Hsp72/73 and c-fos. Results. Corneal opacity was founded at density 0.83 (-6 group), l(-9 group), 1.67(-15 group) Sweek after operation. HSP 72 was expressed in corneal epithelium at the -15 group 24 hours after operation and maximal expression was obtained at 48 hours after operation. Hsp 73 was seen at all group in corneal epithelium and stroma. c-fos was seen in corneal epithelium at ohour after operation in all group. But maximal expression was seen at 24hour after operation in -IS group. Conclusion Corneal opacity was developed at -15D group with statistical significance. Corneal epithelial hyperplasia, increased number of stromal keratocytes, hsp 72 expression and c-fos expression could be significant factors for the corneal opacity.

AB - Purpose. To determine the etiology of the corneal opacity, the author studied on inunnunohistochemical stain for HSP 72/73, c-fos after myopic excimer laser PRK. Methods. Myopic excimer laser PRK was operated on rabbit's cornea to correct -6,-9,-15 diopter of myopia. Comea opacity was examined and immunohistochemical stain was performed for Hsp72/73 and c-fos. Results. Corneal opacity was founded at density 0.83 (-6 group), l(-9 group), 1.67(-15 group) Sweek after operation. HSP 72 was expressed in corneal epithelium at the -15 group 24 hours after operation and maximal expression was obtained at 48 hours after operation. Hsp 73 was seen at all group in corneal epithelium and stroma. c-fos was seen in corneal epithelium at ohour after operation in all group. But maximal expression was seen at 24hour after operation in -IS group. Conclusion Corneal opacity was developed at -15D group with statistical significance. Corneal epithelial hyperplasia, increased number of stromal keratocytes, hsp 72 expression and c-fos expression could be significant factors for the corneal opacity.

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JO - Investigative Ophthalmology and Visual Science

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