The relationship between cytokines and HPV-16, HPV-16 E6, E7, and high-risk HPV viral load in the uterine cervix

Seung Hun Song, Jae Kwan Lee, Oye Sun Seok, Ho Suk Saw

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objectives: To investigate intralesional cytokine levels in precancerous lesions of the uterine cervix and their relationship with human papillomavirus (HPV) type 16, HPV type 16 E6/E7, and high-risk HPV viral load. Methods: We performed a prospective study of 67 patients between May 2005 and December 2005. Hybrid Capture II testing was used to identify patients as high-risk HPV DNA-positive or -negative. HPV DNA Chip test was performed for HPV genotyping in all cases found to be HPV DNA-positive. Real-time PCR was used to quantify HPV-16 E6, E7, interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor α (TNF-α), and interferon γ (IFN-γ) transcripts. Results: Among high-risk HPV-infected women, intralesional TNF-α, IL-6, IL-10, and IFN-γ levels had no significant differences according to histologic grade. In multivariate logistic regression analysis, TNF-α, IL-6, IL-10, and IFN-γ were not associated with HPV-16. Increased IFN-γ was significantly associated with HPV-16 E6- and E7-positive (OR 28.197, 95% CI: 2.658-299.110; OR 19.617, 95% CI: 2.135-180.253, respectively), whereas TNF-α, IL-6, and IL-10 were not associated with HPV-16 E6 and E7. In multiple regression analysis, elevated IFN-γ was significantly associated with increased HPV viral load (P = 0.039), whereas TNF-α, IL-6, and IL-10 were not significantly associated with HPV viral load. Conclusions: Among HPV-infected women, IFN-γ is significantly associated with HPV-16 E6, E7, and high-risk HPV viral load in the uterine cervix. Thus, increased intralesional IFN-γ may be considered to be a prognostic marker for oncogenic potential of high-risk HPV.

Original languageEnglish
Pages (from-to)732-738
Number of pages7
JournalGynecologic Oncology
Volume104
Issue number3
DOIs
Publication statusPublished - 2007 Mar 1

Fingerprint

Human papillomavirus 16
Viral Load
Cervix Uteri
Cytokines
Interleukin-10
Interleukin-6
Human Papillomavirus DNA Tests
Regression Analysis
DNA
Oligonucleotide Array Sequence Analysis
Interferons
Real-Time Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Logistic Models
Prospective Studies

Keywords

  • Human papillomavirus (HPV)
  • Interferon γ (IFN-γ)
  • Interleukin-10 (IL-10)
  • Interleukin-6 (IL-6)
  • Tumor necrosis factor α (TNF-α)

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynaecology

Cite this

The relationship between cytokines and HPV-16, HPV-16 E6, E7, and high-risk HPV viral load in the uterine cervix. / Song, Seung Hun; Lee, Jae Kwan; Seok, Oye Sun; Saw, Ho Suk.

In: Gynecologic Oncology, Vol. 104, No. 3, 01.03.2007, p. 732-738.

Research output: Contribution to journalArticle

@article{d6ac606bb80d421cb00819f2cf34be44,
title = "The relationship between cytokines and HPV-16, HPV-16 E6, E7, and high-risk HPV viral load in the uterine cervix",
abstract = "Objectives: To investigate intralesional cytokine levels in precancerous lesions of the uterine cervix and their relationship with human papillomavirus (HPV) type 16, HPV type 16 E6/E7, and high-risk HPV viral load. Methods: We performed a prospective study of 67 patients between May 2005 and December 2005. Hybrid Capture II testing was used to identify patients as high-risk HPV DNA-positive or -negative. HPV DNA Chip test was performed for HPV genotyping in all cases found to be HPV DNA-positive. Real-time PCR was used to quantify HPV-16 E6, E7, interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor α (TNF-α), and interferon γ (IFN-γ) transcripts. Results: Among high-risk HPV-infected women, intralesional TNF-α, IL-6, IL-10, and IFN-γ levels had no significant differences according to histologic grade. In multivariate logistic regression analysis, TNF-α, IL-6, IL-10, and IFN-γ were not associated with HPV-16. Increased IFN-γ was significantly associated with HPV-16 E6- and E7-positive (OR 28.197, 95{\%} CI: 2.658-299.110; OR 19.617, 95{\%} CI: 2.135-180.253, respectively), whereas TNF-α, IL-6, and IL-10 were not associated with HPV-16 E6 and E7. In multiple regression analysis, elevated IFN-γ was significantly associated with increased HPV viral load (P = 0.039), whereas TNF-α, IL-6, and IL-10 were not significantly associated with HPV viral load. Conclusions: Among HPV-infected women, IFN-γ is significantly associated with HPV-16 E6, E7, and high-risk HPV viral load in the uterine cervix. Thus, increased intralesional IFN-γ may be considered to be a prognostic marker for oncogenic potential of high-risk HPV.",
keywords = "Human papillomavirus (HPV), Interferon γ (IFN-γ), Interleukin-10 (IL-10), Interleukin-6 (IL-6), Tumor necrosis factor α (TNF-α)",
author = "Song, {Seung Hun} and Lee, {Jae Kwan} and Seok, {Oye Sun} and Saw, {Ho Suk}",
year = "2007",
month = "3",
day = "1",
doi = "10.1016/j.ygyno.2006.10.054",
language = "English",
volume = "104",
pages = "732--738",
journal = "Gynecologic Oncology",
issn = "0090-8258",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - The relationship between cytokines and HPV-16, HPV-16 E6, E7, and high-risk HPV viral load in the uterine cervix

AU - Song, Seung Hun

AU - Lee, Jae Kwan

AU - Seok, Oye Sun

AU - Saw, Ho Suk

PY - 2007/3/1

Y1 - 2007/3/1

N2 - Objectives: To investigate intralesional cytokine levels in precancerous lesions of the uterine cervix and their relationship with human papillomavirus (HPV) type 16, HPV type 16 E6/E7, and high-risk HPV viral load. Methods: We performed a prospective study of 67 patients between May 2005 and December 2005. Hybrid Capture II testing was used to identify patients as high-risk HPV DNA-positive or -negative. HPV DNA Chip test was performed for HPV genotyping in all cases found to be HPV DNA-positive. Real-time PCR was used to quantify HPV-16 E6, E7, interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor α (TNF-α), and interferon γ (IFN-γ) transcripts. Results: Among high-risk HPV-infected women, intralesional TNF-α, IL-6, IL-10, and IFN-γ levels had no significant differences according to histologic grade. In multivariate logistic regression analysis, TNF-α, IL-6, IL-10, and IFN-γ were not associated with HPV-16. Increased IFN-γ was significantly associated with HPV-16 E6- and E7-positive (OR 28.197, 95% CI: 2.658-299.110; OR 19.617, 95% CI: 2.135-180.253, respectively), whereas TNF-α, IL-6, and IL-10 were not associated with HPV-16 E6 and E7. In multiple regression analysis, elevated IFN-γ was significantly associated with increased HPV viral load (P = 0.039), whereas TNF-α, IL-6, and IL-10 were not significantly associated with HPV viral load. Conclusions: Among HPV-infected women, IFN-γ is significantly associated with HPV-16 E6, E7, and high-risk HPV viral load in the uterine cervix. Thus, increased intralesional IFN-γ may be considered to be a prognostic marker for oncogenic potential of high-risk HPV.

AB - Objectives: To investigate intralesional cytokine levels in precancerous lesions of the uterine cervix and their relationship with human papillomavirus (HPV) type 16, HPV type 16 E6/E7, and high-risk HPV viral load. Methods: We performed a prospective study of 67 patients between May 2005 and December 2005. Hybrid Capture II testing was used to identify patients as high-risk HPV DNA-positive or -negative. HPV DNA Chip test was performed for HPV genotyping in all cases found to be HPV DNA-positive. Real-time PCR was used to quantify HPV-16 E6, E7, interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor α (TNF-α), and interferon γ (IFN-γ) transcripts. Results: Among high-risk HPV-infected women, intralesional TNF-α, IL-6, IL-10, and IFN-γ levels had no significant differences according to histologic grade. In multivariate logistic regression analysis, TNF-α, IL-6, IL-10, and IFN-γ were not associated with HPV-16. Increased IFN-γ was significantly associated with HPV-16 E6- and E7-positive (OR 28.197, 95% CI: 2.658-299.110; OR 19.617, 95% CI: 2.135-180.253, respectively), whereas TNF-α, IL-6, and IL-10 were not associated with HPV-16 E6 and E7. In multiple regression analysis, elevated IFN-γ was significantly associated with increased HPV viral load (P = 0.039), whereas TNF-α, IL-6, and IL-10 were not significantly associated with HPV viral load. Conclusions: Among HPV-infected women, IFN-γ is significantly associated with HPV-16 E6, E7, and high-risk HPV viral load in the uterine cervix. Thus, increased intralesional IFN-γ may be considered to be a prognostic marker for oncogenic potential of high-risk HPV.

KW - Human papillomavirus (HPV)

KW - Interferon γ (IFN-γ)

KW - Interleukin-10 (IL-10)

KW - Interleukin-6 (IL-6)

KW - Tumor necrosis factor α (TNF-α)

UR - http://www.scopus.com/inward/record.url?scp=33846908537&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846908537&partnerID=8YFLogxK

U2 - 10.1016/j.ygyno.2006.10.054

DO - 10.1016/j.ygyno.2006.10.054

M3 - Article

C2 - 17188341

AN - SCOPUS:33846908537

VL - 104

SP - 732

EP - 738

JO - Gynecologic Oncology

JF - Gynecologic Oncology

SN - 0090-8258

IS - 3

ER -