The relationship between cytokines and HPV-16, HPV-16 E6, E7, and high-risk HPV viral load in the uterine cervix

Seung Hun Song, Jae Kwan Lee, Oye Sun Seok, Ho Suk Saw

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Objectives: To investigate intralesional cytokine levels in precancerous lesions of the uterine cervix and their relationship with human papillomavirus (HPV) type 16, HPV type 16 E6/E7, and high-risk HPV viral load. Methods: We performed a prospective study of 67 patients between May 2005 and December 2005. Hybrid Capture II testing was used to identify patients as high-risk HPV DNA-positive or -negative. HPV DNA Chip test was performed for HPV genotyping in all cases found to be HPV DNA-positive. Real-time PCR was used to quantify HPV-16 E6, E7, interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor α (TNF-α), and interferon γ (IFN-γ) transcripts. Results: Among high-risk HPV-infected women, intralesional TNF-α, IL-6, IL-10, and IFN-γ levels had no significant differences according to histologic grade. In multivariate logistic regression analysis, TNF-α, IL-6, IL-10, and IFN-γ were not associated with HPV-16. Increased IFN-γ was significantly associated with HPV-16 E6- and E7-positive (OR 28.197, 95% CI: 2.658-299.110; OR 19.617, 95% CI: 2.135-180.253, respectively), whereas TNF-α, IL-6, and IL-10 were not associated with HPV-16 E6 and E7. In multiple regression analysis, elevated IFN-γ was significantly associated with increased HPV viral load (P = 0.039), whereas TNF-α, IL-6, and IL-10 were not significantly associated with HPV viral load. Conclusions: Among HPV-infected women, IFN-γ is significantly associated with HPV-16 E6, E7, and high-risk HPV viral load in the uterine cervix. Thus, increased intralesional IFN-γ may be considered to be a prognostic marker for oncogenic potential of high-risk HPV.

Original languageEnglish
Pages (from-to)732-738
Number of pages7
JournalGynecologic Oncology
Volume104
Issue number3
DOIs
Publication statusPublished - 2007 Mar

Keywords

  • Human papillomavirus (HPV)
  • Interferon γ (IFN-γ)
  • Interleukin-10 (IL-10)
  • Interleukin-6 (IL-6)
  • Tumor necrosis factor α (TNF-α)

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynaecology

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