The reprogramming factor nuclear receptor subfamily 5, group A, member 2 cannot replace octamer-binding transcription factor 4 function in the self-renewal of embryonic stem cells

Kyeng Won Choi, Hye Rim Oh, Jaeyoung Lee, Bobae Lim, Yong Mahn Han, Jun Seo Oh, Jungho Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Although octamer-binding transcription factor 4 (Oct-4) is one of the most intensively studied factors in mammalian development, no cellular genes capable of replacing Oct-4 function in embryonic stem (ES) cells have been found. Recent data show that nuclear receptor subfamily 5, group A, member 2 (Nr5a2) is able to replace Oct-4 function in the reprogramming process; however, it is unclear whether Nr5a2 can replace Oct-4 function in ES cells. In this study, the ability of Nr5a2 to maintain self-renewal and pluripotency in ES cells was investigated. Nr5a2 localized to the nucleus in ES cells, similarly to Oct-4. However, expression of Nr5a2 failed to rescue the stem cell phenotype or to maintain the self-renewal ability of ES cells. Furthermore, as compared with Oct-4-expressing ES cells, Nr5a2-expressing ES cells showed a reduced number of cells in S-phase, did not expand normally, and did not remain in an undifferentiated state. Ectopic expression of Nr5a2 in ES cells was not able to activate transcription of ES cell-specific genes, and gene expression profiling demonstrated differences between Nr5a2-expressing and Oct-4-expressing ES cells. In addition, Nr5a2-expressing ES cells were not able to form teratomas in nude mice. Taken together, these results strongly suggest that the gene regulation properties of Nr5a2 and Oct-4 and their abilities to confer self-renewal and pluripotency of ES cells differ. The present study provides strong evidence that Nr5a2 cannot replace Oct-4 function in ES cells.

Original languageEnglish
Pages (from-to)1029-1045
Number of pages17
JournalFEBS Journal
Volume281
Issue number4
DOIs
Publication statusPublished - 2014 Feb 1

Fingerprint

Octamer Transcription Factors
Embryonic Stem Cells
Cytoplasmic and Nuclear Receptors
Stem cells
Transcription Factors
Gene expression
Genes
Teratoma
Gene Expression Profiling

Keywords

  • embryonic stem cell
  • induced pluripotent stem cell
  • nuclear receptor subfamily 5, group A, member 2 (Nr5a2)
  • octamer-binding transcription factor 4 (Oct-4)
  • self-renewal

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The reprogramming factor nuclear receptor subfamily 5, group A, member 2 cannot replace octamer-binding transcription factor 4 function in the self-renewal of embryonic stem cells. / Choi, Kyeng Won; Oh, Hye Rim; Lee, Jaeyoung; Lim, Bobae; Han, Yong Mahn; Oh, Jun Seo; Kim, Jungho.

In: FEBS Journal, Vol. 281, No. 4, 01.02.2014, p. 1029-1045.

Research output: Contribution to journalArticle

Choi, Kyeng Won ; Oh, Hye Rim ; Lee, Jaeyoung ; Lim, Bobae ; Han, Yong Mahn ; Oh, Jun Seo ; Kim, Jungho. / The reprogramming factor nuclear receptor subfamily 5, group A, member 2 cannot replace octamer-binding transcription factor 4 function in the self-renewal of embryonic stem cells. In: FEBS Journal. 2014 ; Vol. 281, No. 4. pp. 1029-1045.
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AB - Although octamer-binding transcription factor 4 (Oct-4) is one of the most intensively studied factors in mammalian development, no cellular genes capable of replacing Oct-4 function in embryonic stem (ES) cells have been found. Recent data show that nuclear receptor subfamily 5, group A, member 2 (Nr5a2) is able to replace Oct-4 function in the reprogramming process; however, it is unclear whether Nr5a2 can replace Oct-4 function in ES cells. In this study, the ability of Nr5a2 to maintain self-renewal and pluripotency in ES cells was investigated. Nr5a2 localized to the nucleus in ES cells, similarly to Oct-4. However, expression of Nr5a2 failed to rescue the stem cell phenotype or to maintain the self-renewal ability of ES cells. Furthermore, as compared with Oct-4-expressing ES cells, Nr5a2-expressing ES cells showed a reduced number of cells in S-phase, did not expand normally, and did not remain in an undifferentiated state. Ectopic expression of Nr5a2 in ES cells was not able to activate transcription of ES cell-specific genes, and gene expression profiling demonstrated differences between Nr5a2-expressing and Oct-4-expressing ES cells. In addition, Nr5a2-expressing ES cells were not able to form teratomas in nude mice. Taken together, these results strongly suggest that the gene regulation properties of Nr5a2 and Oct-4 and their abilities to confer self-renewal and pluripotency of ES cells differ. The present study provides strong evidence that Nr5a2 cannot replace Oct-4 function in ES cells.

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