TY - JOUR
T1 - The role of 14-3-3β in transcriptional activation of estrogen receptor α and its involvement in proliferation of breast cancer cells
AU - Kim, Yoonseo
AU - Kim, Hyungjin
AU - Jang, Sung Wuk
AU - Ko, Jesang
N1 - Funding Information:
This work was supported by the Basic Science Research Program ( 2011-0017957 ), the Basic Research Laboratory Program ( 2011-0001573 ), and the Bio & Medical Technology Development Program ( 2011-0027756 ) from the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology, Republic of Korea.
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/10/14
Y1 - 2011/10/14
N2 - The estrogen receptor (ER) functions as a transcription factor that mediates the effects of estrogen. ERα, which plays a crucial role in the development and progression of breast cancer, is activated by estrogen binding, leading to receptor phosphorylation, dimerization, and recruitment of co-activators and chaperons to the estrogen-bound receptor complex. The 14-3-3 proteins bind to target proteins via phosphorylation and influence many cellular events by altering their subcellular localization or acting as a chaperone. However, regulation of ERα expression and transactivation by the 14-3-3 proteins has not been reported. We demonstrate that 14-3-3β functions as a positive regulator of ERα through a direct protein-protein interaction in an estrogen-dependent manner. Ectopic expression of 14-3-3β stimulated ERα-mediated transcriptional activity in MCF-7 breast cancer cells. Enhanced ERα transcriptional activity due to 14-3-3β increased the expressions of the endogenous ERα target genes, leading to proliferation of breast cancer cells. We suggest that 14-3-3β has oncogenic potential in breast cancer via binding to ERα and activation of the transcriptional activity of ERα.
AB - The estrogen receptor (ER) functions as a transcription factor that mediates the effects of estrogen. ERα, which plays a crucial role in the development and progression of breast cancer, is activated by estrogen binding, leading to receptor phosphorylation, dimerization, and recruitment of co-activators and chaperons to the estrogen-bound receptor complex. The 14-3-3 proteins bind to target proteins via phosphorylation and influence many cellular events by altering their subcellular localization or acting as a chaperone. However, regulation of ERα expression and transactivation by the 14-3-3 proteins has not been reported. We demonstrate that 14-3-3β functions as a positive regulator of ERα through a direct protein-protein interaction in an estrogen-dependent manner. Ectopic expression of 14-3-3β stimulated ERα-mediated transcriptional activity in MCF-7 breast cancer cells. Enhanced ERα transcriptional activity due to 14-3-3β increased the expressions of the endogenous ERα target genes, leading to proliferation of breast cancer cells. We suggest that 14-3-3β has oncogenic potential in breast cancer via binding to ERα and activation of the transcriptional activity of ERα.
KW - 14-3-3
KW - Breast cancer
KW - Estrogen receptor
UR - http://www.scopus.com/inward/record.url?scp=80054077852&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80054077852&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2011.09.056
DO - 10.1016/j.bbrc.2011.09.056
M3 - Article
C2 - 21946067
AN - SCOPUS:80054077852
VL - 414
SP - 199
EP - 204
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -
