Abstract
The estrogen receptor (ER) functions as a transcription factor that mediates the effects of estrogen. ERα, which plays a crucial role in the development and progression of breast cancer, is activated by estrogen binding, leading to receptor phosphorylation, dimerization, and recruitment of co-activators and chaperons to the estrogen-bound receptor complex. The 14-3-3 proteins bind to target proteins via phosphorylation and influence many cellular events by altering their subcellular localization or acting as a chaperone. However, regulation of ERα expression and transactivation by the 14-3-3 proteins has not been reported. We demonstrate that 14-3-3β functions as a positive regulator of ERα through a direct protein-protein interaction in an estrogen-dependent manner. Ectopic expression of 14-3-3β stimulated ERα-mediated transcriptional activity in MCF-7 breast cancer cells. Enhanced ERα transcriptional activity due to 14-3-3β increased the expressions of the endogenous ERα target genes, leading to proliferation of breast cancer cells. We suggest that 14-3-3β has oncogenic potential in breast cancer via binding to ERα and activation of the transcriptional activity of ERα.
| Original language | English |
|---|---|
| Pages (from-to) | 199-204 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 414 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2011 Oct 14 |
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Keywords
- 14-3-3
- Breast cancer
- Estrogen receptor
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Molecular Biology
Cite this
The role of 14-3-3β in transcriptional activation of estrogen receptor α and its involvement in proliferation of breast cancer cells. / Kim, Yoonseo; Kim, Hyungjin; Jang, Sung Wuk; Ko, Je Sang.
In: Biochemical and Biophysical Research Communications, Vol. 414, No. 1, 14.10.2011, p. 199-204.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The role of 14-3-3β in transcriptional activation of estrogen receptor α and its involvement in proliferation of breast cancer cells
AU - Kim, Yoonseo
AU - Kim, Hyungjin
AU - Jang, Sung Wuk
AU - Ko, Je Sang
PY - 2011/10/14
Y1 - 2011/10/14
N2 - The estrogen receptor (ER) functions as a transcription factor that mediates the effects of estrogen. ERα, which plays a crucial role in the development and progression of breast cancer, is activated by estrogen binding, leading to receptor phosphorylation, dimerization, and recruitment of co-activators and chaperons to the estrogen-bound receptor complex. The 14-3-3 proteins bind to target proteins via phosphorylation and influence many cellular events by altering their subcellular localization or acting as a chaperone. However, regulation of ERα expression and transactivation by the 14-3-3 proteins has not been reported. We demonstrate that 14-3-3β functions as a positive regulator of ERα through a direct protein-protein interaction in an estrogen-dependent manner. Ectopic expression of 14-3-3β stimulated ERα-mediated transcriptional activity in MCF-7 breast cancer cells. Enhanced ERα transcriptional activity due to 14-3-3β increased the expressions of the endogenous ERα target genes, leading to proliferation of breast cancer cells. We suggest that 14-3-3β has oncogenic potential in breast cancer via binding to ERα and activation of the transcriptional activity of ERα.
AB - The estrogen receptor (ER) functions as a transcription factor that mediates the effects of estrogen. ERα, which plays a crucial role in the development and progression of breast cancer, is activated by estrogen binding, leading to receptor phosphorylation, dimerization, and recruitment of co-activators and chaperons to the estrogen-bound receptor complex. The 14-3-3 proteins bind to target proteins via phosphorylation and influence many cellular events by altering their subcellular localization or acting as a chaperone. However, regulation of ERα expression and transactivation by the 14-3-3 proteins has not been reported. We demonstrate that 14-3-3β functions as a positive regulator of ERα through a direct protein-protein interaction in an estrogen-dependent manner. Ectopic expression of 14-3-3β stimulated ERα-mediated transcriptional activity in MCF-7 breast cancer cells. Enhanced ERα transcriptional activity due to 14-3-3β increased the expressions of the endogenous ERα target genes, leading to proliferation of breast cancer cells. We suggest that 14-3-3β has oncogenic potential in breast cancer via binding to ERα and activation of the transcriptional activity of ERα.
KW - 14-3-3
KW - Breast cancer
KW - Estrogen receptor
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UR - http://www.scopus.com/inward/citedby.url?scp=80054077852&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2011.09.056
DO - 10.1016/j.bbrc.2011.09.056
M3 - Article
VL - 414
SP - 199
EP - 204
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 1
ER -