The role of 14-3-3β in transcriptional activation of estrogen receptor α and its involvement in proliferation of breast cancer cells

Yoonseo Kim, Hyungjin Kim, Sung Wuk Jang, Jesang Ko

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The estrogen receptor (ER) functions as a transcription factor that mediates the effects of estrogen. ERα, which plays a crucial role in the development and progression of breast cancer, is activated by estrogen binding, leading to receptor phosphorylation, dimerization, and recruitment of co-activators and chaperons to the estrogen-bound receptor complex. The 14-3-3 proteins bind to target proteins via phosphorylation and influence many cellular events by altering their subcellular localization or acting as a chaperone. However, regulation of ERα expression and transactivation by the 14-3-3 proteins has not been reported. We demonstrate that 14-3-3β functions as a positive regulator of ERα through a direct protein-protein interaction in an estrogen-dependent manner. Ectopic expression of 14-3-3β stimulated ERα-mediated transcriptional activity in MCF-7 breast cancer cells. Enhanced ERα transcriptional activity due to 14-3-3β increased the expressions of the endogenous ERα target genes, leading to proliferation of breast cancer cells. We suggest that 14-3-3β has oncogenic potential in breast cancer via binding to ERα and activation of the transcriptional activity of ERα.

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalBiochemical and biophysical research communications
Volume414
Issue number1
DOIs
Publication statusPublished - 2011 Oct 14

    Fingerprint

Keywords

  • 14-3-3
  • Breast cancer
  • Estrogen receptor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this