TY - JOUR
T1 - The role of clusterin in retinal development and free radical damage
AU - Kim, Jeong Hun
AU - Kim, Jin Hyoung
AU - Yu, Young Suk
AU - Min, Bon Hong
AU - Kim, Kyu Won
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/11
Y1 - 2007/11
N2 - Aim: To assess the role of clusterin in retinal vascular development and in free radical damage in vivo and in vitro. Methods: The expression of clusterin, von Willebrand factor (vWF), flk-1, heat shock protein 27 (Hsp27) and heat shock protein 70 (Hsp70) was examined in the retinas of developing mice and oxygen-induced retinopathy (OIR) mice by immunofluorescence staining and western blot analysis. Hydrogen peroxide (H2O2)-pretreated human retinal endothelial cells (HREC) and astrocytes were cultured in the presence or absence of exogenous clusterin, and then the cell viability was measured using the MTT assay and DAPI staining. Results: Clusterin was expressed mainly in the inner retina and co-localised with vWF, an endothelial cell marker. During the mouse developmental process, clusterin expression was decreased, which was similar to the expression of Rk-1, vWF and Hsp27. Furthermore, in the OIR model, clusterin expression changed in a similar way to both vWF and Hsp27. Under hypoxic conditions, clusterin expression increased in HREC and astrocytes. In H2O2-pretreated HREC and astrocytes, clusterin protected against apoptotic cell death. Conclusions: These results suggest that clusterin is associated with protection from apoptotic retinal cell death in retinal development and in free radical damage.
AB - Aim: To assess the role of clusterin in retinal vascular development and in free radical damage in vivo and in vitro. Methods: The expression of clusterin, von Willebrand factor (vWF), flk-1, heat shock protein 27 (Hsp27) and heat shock protein 70 (Hsp70) was examined in the retinas of developing mice and oxygen-induced retinopathy (OIR) mice by immunofluorescence staining and western blot analysis. Hydrogen peroxide (H2O2)-pretreated human retinal endothelial cells (HREC) and astrocytes were cultured in the presence or absence of exogenous clusterin, and then the cell viability was measured using the MTT assay and DAPI staining. Results: Clusterin was expressed mainly in the inner retina and co-localised with vWF, an endothelial cell marker. During the mouse developmental process, clusterin expression was decreased, which was similar to the expression of Rk-1, vWF and Hsp27. Furthermore, in the OIR model, clusterin expression changed in a similar way to both vWF and Hsp27. Under hypoxic conditions, clusterin expression increased in HREC and astrocytes. In H2O2-pretreated HREC and astrocytes, clusterin protected against apoptotic cell death. Conclusions: These results suggest that clusterin is associated with protection from apoptotic retinal cell death in retinal development and in free radical damage.
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U2 - 10.1136/bjo.2007.115220
DO - 10.1136/bjo.2007.115220
M3 - Article
C2 - 17475708
AN - SCOPUS:35748939116
VL - 91
SP - 1541
EP - 1546
JO - British Journal of Ophthalmology
JF - British Journal of Ophthalmology
SN - 0007-1161
IS - 11
ER -