The role of disulfide bond isomerase A (DsbA) of Escherichia coli O157: H7 in biofilm formation and virulence

Yunho Lee, Younghoon Kim, Sujin Yeom, Sae Hun Kim, Sungsu Park, Che Ok Jeon, Woojun Park

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The role of periplasmic disulfide oxidoreductase DsbA in Shiga toxin-producing Escherichia coli O157:H7 (STEC) was investigated. Deletion of dsbA (ΔdsbA) significantly decreased cell motility and alkaline phosphatase activity in STEC. STEC ΔdsbA also showed greater sensitivity to menadione and under low pH conditions. Significant reductions in surface attachment to both biotic (HT-29 epithelial cells) and abiotic (polystyrene and polyvinyl chloride) surfaces were observed in STEC ΔdsbA. In addition, no biofilm formation was detected in STEC ΔdsbA compared to wild-type cells in glass capillary tubes under continuous flow-culture system conditions. In the nematode model Caenorhabditis elegans-killing assay, the deletion of dsbA in STEC resulted in attenuated virulence compared to wild-type cells. STEC ΔdsbA was also found to have a reduced ability to colonize the nematode gut. These results suggest that DsbA plays important roles in biofilm formation and virulence in STEC cells.

Original languageEnglish
Pages (from-to)213-222
Number of pages10
JournalFEMS Microbiology Letters
Volume278
Issue number2
DOIs
Publication statusPublished - 2008 Jan 1

Fingerprint

Protein Disulfide-Isomerases
Shiga-Toxigenic Escherichia coli
Escherichia coli O157
Biofilms
Virulence
Vitamin K 3
HT29 Cells
Polystyrenes
Caenorhabditis elegans
Polyvinyl Chloride
Disulfides
Cell Movement
Glass
Alkaline Phosphatase
Epithelial Cells

Keywords

  • Biofilm
  • Caenorhabditis elegans
  • Disulfide bond
  • Virulence

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

The role of disulfide bond isomerase A (DsbA) of Escherichia coli O157 : H7 in biofilm formation and virulence. / Lee, Yunho; Kim, Younghoon; Yeom, Sujin; Kim, Sae Hun; Park, Sungsu; Jeon, Che Ok; Park, Woojun.

In: FEMS Microbiology Letters, Vol. 278, No. 2, 01.01.2008, p. 213-222.

Research output: Contribution to journalArticle

Lee, Yunho ; Kim, Younghoon ; Yeom, Sujin ; Kim, Sae Hun ; Park, Sungsu ; Jeon, Che Ok ; Park, Woojun. / The role of disulfide bond isomerase A (DsbA) of Escherichia coli O157 : H7 in biofilm formation and virulence. In: FEMS Microbiology Letters. 2008 ; Vol. 278, No. 2. pp. 213-222.
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