The role of nitric oxide in vivo feline erection under hypoxia

Je-Jong Kim, Du Geon Moon, S. K. Koh

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Previous in vitro studies have demonstrated that the cavernous relaxation under hypoxia does not involve the endothelium dependent mechanism. However, the mechanism of nitric oxide pathway under hypoxia are not fully evaluated or understood yet in vivo. The changes of intracavernous pressure to various vasoactive substances were monitored in 45 mature male cats in vivo under normoxia and hypoxia (pH:7.03, PO2:25.52 mmHg, PCO2: 84.66 mmHg). L-arginine and SNAP (s-nitroso-n-acetyl-penicillamine) produced cavernous relaxation under normoxia, but not under hypoxia (n = 19, P < 0.01). The L-arginine-induced relaxations were inhibited by L-NAME (Nω-nitro-1-arginine-methyl-ester) or methylene blue under normoxia (n = 19, P < 0.01). The cavernous relaxation was 58% suppressed under hypoxia compared to normoxia with 10-3 M/0.2 ml of acetylcholine (n = 22, P < 0.01). Moreover, L-NAME attenuated the acetylcholine-induced relaxation under normoxia, but not under hypoxia (n = 22, P < 0.05). Epinephrine suppressed the acetylcholine-induced relaxation in both conditions (n = 10, P < 0.01), while indomethacin significantly potentiated the acetylcholine-induced relaxation under normoxia compared to hypoxia (n = 6, P < 0.05). However, none of these substances responded in severe hypoxia (PO2 < 15 mmHg, n = 3). These results suggest that erectile and contractile responses are attenuated under hypoxia. The endothelium derived relaxation via nitric oxide does not play a role in cavernous relaxation under definitive hypoxia with acidosis like in ischemic priapism (PO2 < 30 mmHg, pH < 7.25).

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalInternational Journal of Impotence Research
Volume10
Issue number3
Publication statusPublished - 1998 Oct 13

Fingerprint

Felidae
Nitric Oxide
Acetylcholine
NG-Nitroarginine Methyl Ester
Endothelium
Arginine
Hypoxia
Priapism
Penicillamine
Methylene Blue
Acidosis
Indomethacin
Epinephrine
Cats
Pressure

Keywords

  • EDRF
  • Hypoxia
  • In vivo
  • Priapism

ASJC Scopus subject areas

  • Urology

Cite this

The role of nitric oxide in vivo feline erection under hypoxia. / Kim, Je-Jong; Moon, Du Geon; Koh, S. K.

In: International Journal of Impotence Research, Vol. 10, No. 3, 13.10.1998, p. 145-151.

Research output: Contribution to journalArticle

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