The role of the CDH1 promoter hypermethylation in the axillary lymph node metastasis and prognosis

Seung Pil Jung, Sangmin Kim, Seok Jin Nam, Insun Kim, Jeoung Won Bae

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: Hypermethylation of the tumor suppressor genes is frequently observed in the tumor development and progression. However, the correlation between the hypermethylation of the tumor suppressor genes, CDH1 and the axillary lymph node (ALN) metastasis is not fully elucidated. To verify the role of the CDH1 promoter hypermethylation in the ALN metastasis and prognosis, we compared the methylation status of the CDH1 genes in the primary lesion and the paired metastatic ALNs. Methods: We selected a total of 122 paraffin-embedded specimens of the primary and paired metastatic lymph node from 61 breast cancer patients and analyzed the frequency of hypermethylation in the primary and metastatic lymph node using the methylation-specific polymerase chain reaction. In addition, the methylation status of CDH1 was analyzed with the clinicopathologic characteristics, the disease-free survival and disease-specific survival. Results: The hypermethylation of CDH1 gene was identified in 54 (88.5%) of the 61 patients who had axillary metastasis. The hypermethylation status of the CDH1 gene was significantly increased in the metastatic ALNs compared with that in the primary tumors (60.7% vs. 45.9%, p<0.001). The hypermethylation status of the CDH1 genes in the metastatic ALNs was associated with a poor histologic grade (p=0.041) and the patients who had methylated tumor in the primary lesion showed worse disease-free survival than the patients who did not have methylated tumor (p=0.046). Conclusion: This study suggests that hypermethylation of the CDH1 gene may play a pivotal role in the metastasis of the axillary lymph node and the breast cancer recurrence.

Original languageEnglish
Pages (from-to)16-22
Number of pages7
JournalJournal of Breast Cancer
Volume16
Issue number1
DOIs
Publication statusPublished - 2013 Mar 1

Fingerprint

Lymph Nodes
Neoplasm Metastasis
Methylation
Genes
Tumor Suppressor Genes
Disease-Free Survival
Neoplasms
Breast Neoplasms
Paraffin
Recurrence
Polymerase Chain Reaction
Survival

Keywords

  • Breast neoplasms
  • Lymph nodes
  • Methylation
  • Recurrence
  • Tumor suppressor genes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

The role of the CDH1 promoter hypermethylation in the axillary lymph node metastasis and prognosis. / Jung, Seung Pil; Kim, Sangmin; Nam, Seok Jin; Kim, Insun; Bae, Jeoung Won.

In: Journal of Breast Cancer, Vol. 16, No. 1, 01.03.2013, p. 16-22.

Research output: Contribution to journalArticle

Jung, Seung Pil ; Kim, Sangmin ; Nam, Seok Jin ; Kim, Insun ; Bae, Jeoung Won. / The role of the CDH1 promoter hypermethylation in the axillary lymph node metastasis and prognosis. In: Journal of Breast Cancer. 2013 ; Vol. 16, No. 1. pp. 16-22.
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abstract = "Purpose: Hypermethylation of the tumor suppressor genes is frequently observed in the tumor development and progression. However, the correlation between the hypermethylation of the tumor suppressor genes, CDH1 and the axillary lymph node (ALN) metastasis is not fully elucidated. To verify the role of the CDH1 promoter hypermethylation in the ALN metastasis and prognosis, we compared the methylation status of the CDH1 genes in the primary lesion and the paired metastatic ALNs. Methods: We selected a total of 122 paraffin-embedded specimens of the primary and paired metastatic lymph node from 61 breast cancer patients and analyzed the frequency of hypermethylation in the primary and metastatic lymph node using the methylation-specific polymerase chain reaction. In addition, the methylation status of CDH1 was analyzed with the clinicopathologic characteristics, the disease-free survival and disease-specific survival. Results: The hypermethylation of CDH1 gene was identified in 54 (88.5{\%}) of the 61 patients who had axillary metastasis. The hypermethylation status of the CDH1 gene was significantly increased in the metastatic ALNs compared with that in the primary tumors (60.7{\%} vs. 45.9{\%}, p<0.001). The hypermethylation status of the CDH1 genes in the metastatic ALNs was associated with a poor histologic grade (p=0.041) and the patients who had methylated tumor in the primary lesion showed worse disease-free survival than the patients who did not have methylated tumor (p=0.046). Conclusion: This study suggests that hypermethylation of the CDH1 gene may play a pivotal role in the metastasis of the axillary lymph node and the breast cancer recurrence.",
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