The Role of the FRE Family of Plasma Membrane Reductases in the Uptake of Siderophore-Iron in Saccharomyces cerevisiae

Cheol-Won Yun, Matthew Bauler, Robert E. Moore, Phillip E. Klebba, Caroline C. Philpott

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Saccharomyces cerevisiae takes up siderophore-bound iron through two distinct systems, one that requires siderophore transporters of the ARN family and one that requires the high affinity ferrous iron transporter on the plasma membrane. Uptake through the plasma membrane ferrous iron transporter requires that the iron first must dissociate from the siderophore and undergo reduction to the ferrous form. FRE1 and FRE2 encode cell surface metalloreductases that are required for reduction and uptake of free ferric iron. The yeast genome contains five additional FRE1 and FRE2 homologues, four of which are regulated by iron and the major iron-dependent transcription factor, Aft1p, but whose function remains unknown. Fre3p was required for the reduction and uptake of ferrioxamine B-iron and for growth on ferrioxamine B, ferrichrome, triacetyl-fusarinine C, and rhodotorulic acid in the absence of Fre1p and Fre2p. By indirect immunofluorescence, Fre3p was expressed on the plasma membrane in a pattern similar to that of Fet3p, a component of the high affinity ferrous transporter. Enterobactin, a catecholate siderophore, was not a substrate for Fre3p, and reductive uptake required either Fre1p or Fre2p. Fre4p could facilitate utilization of rhodotorulic acid-iron when the siderophore was present in higher concentrations. We propose that Fre3p and Fre4p are siderophore-iron reductases and that the apparent redundancy of the FRE genes confers the capacity to utilize iron from a variety of siderophore sources.

Original languageEnglish
Pages (from-to)10218-10223
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number13
DOIs
Publication statusPublished - 2001 Mar 30
Externally publishedYes

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Siderophores
Cell membranes
Yeast
Saccharomyces cerevisiae
Oxidoreductases
Iron
Cell Membrane
Ferrichrome
Enterobactin
Genes
Indirect Fluorescent Antibody Technique
Redundancy
Transcription Factors
Yeasts
Genome

ASJC Scopus subject areas

  • Biochemistry

Cite this

The Role of the FRE Family of Plasma Membrane Reductases in the Uptake of Siderophore-Iron in Saccharomyces cerevisiae. / Yun, Cheol-Won; Bauler, Matthew; Moore, Robert E.; Klebba, Phillip E.; Philpott, Caroline C.

In: Journal of Biological Chemistry, Vol. 276, No. 13, 30.03.2001, p. 10218-10223.

Research output: Contribution to journalArticle

Yun, Cheol-Won ; Bauler, Matthew ; Moore, Robert E. ; Klebba, Phillip E. ; Philpott, Caroline C. / The Role of the FRE Family of Plasma Membrane Reductases in the Uptake of Siderophore-Iron in Saccharomyces cerevisiae. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 13. pp. 10218-10223.
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