The roles of HOXB7 in promoting migration, invasion, and anti-apoptosis in gastric cancer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background and Aim: The aim of this study was to compare HOXB7 expression level between gastric cancer and non-cancerous gastric tissues. Additionally, the functional effects of HOXB7, including its pro-migration or invasion and anti-apoptosis roles, were evaluated in gastric cancer cells. Methods: Both gene and protein expression levels of HOXB7 were examined in gastric cancer cell lines, and HOXB7 expression was compared between primary or metastatic gastric cancer tissues and chronic gastritis or intestinal metaplasia tissues. Functional studies included a wound healing assay, a Matrigel invasion assay, and an Annexin-V assay were performed, and Akt/PTEN activity was measured by western blotting. Results: Both gene and protein expression levels of HOXB7 could be clearly detected in various gastric cancer cell lines except MKN-28 cell. HOXB7 expression was significantly higher in primary or metastatic gastric cancer tissues than in chronic gastritis or intestinal metaplasia tissues. HOXB7 knockdown led to inhibition of cell invasion and migration, had an apoptotic effect, downregulated phosphor-Akt, and upregulated PTEN in AGS and SNU-638 cells. Reinforced expression of HOXB7 caused the opposite effects in MKN-28 and MKN-45 cells. Conclusion: Our study suggests that HOXB7 has an oncogenic role in gastric cancer, which might be related to the modulation of Akt/PTEN activity to induce cell migration/invasion and anti-apoptotic effects.

Original languageEnglish
Pages (from-to)1717-1726
Number of pages10
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume31
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1

Fingerprint

Stomach Neoplasms
Apoptosis
Metaplasia
Gastritis
Cell Migration Inhibition
Gene Expression
Cell Line
Annexin A5
Wound Healing
Cell Movement
Stomach
Proteins
Down-Regulation
Western Blotting

Keywords

  • Akt
  • HOXB7
  • PTEN
  • stomach cancer

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

@article{7e5f8ab652aa478f922f4577241f8de9,
title = "The roles of HOXB7 in promoting migration, invasion, and anti-apoptosis in gastric cancer",
abstract = "Background and Aim: The aim of this study was to compare HOXB7 expression level between gastric cancer and non-cancerous gastric tissues. Additionally, the functional effects of HOXB7, including its pro-migration or invasion and anti-apoptosis roles, were evaluated in gastric cancer cells. Methods: Both gene and protein expression levels of HOXB7 were examined in gastric cancer cell lines, and HOXB7 expression was compared between primary or metastatic gastric cancer tissues and chronic gastritis or intestinal metaplasia tissues. Functional studies included a wound healing assay, a Matrigel invasion assay, and an Annexin-V assay were performed, and Akt/PTEN activity was measured by western blotting. Results: Both gene and protein expression levels of HOXB7 could be clearly detected in various gastric cancer cell lines except MKN-28 cell. HOXB7 expression was significantly higher in primary or metastatic gastric cancer tissues than in chronic gastritis or intestinal metaplasia tissues. HOXB7 knockdown led to inhibition of cell invasion and migration, had an apoptotic effect, downregulated phosphor-Akt, and upregulated PTEN in AGS and SNU-638 cells. Reinforced expression of HOXB7 caused the opposite effects in MKN-28 and MKN-45 cells. Conclusion: Our study suggests that HOXB7 has an oncogenic role in gastric cancer, which might be related to the modulation of Akt/PTEN activity to induce cell migration/invasion and anti-apoptotic effects.",
keywords = "Akt, HOXB7, PTEN, stomach cancer",
author = "Joo, {Moon Kyung} and Park, {Jong Jae} and Yoo, {Hyo Soon} and Beomjae Lee and Hoon-Jai Chun and Lee, {Sang Woo} and Young-Tae Bak",
year = "2016",
month = "10",
day = "1",
doi = "10.1111/jgh.13330",
language = "English",
volume = "31",
pages = "1717--1726",
journal = "Journal of Gastroenterology and Hepatology (Australia)",
issn = "0815-9319",
publisher = "Wiley-Blackwell",
number = "10",

}

TY - JOUR

T1 - The roles of HOXB7 in promoting migration, invasion, and anti-apoptosis in gastric cancer

AU - Joo, Moon Kyung

AU - Park, Jong Jae

AU - Yoo, Hyo Soon

AU - Lee, Beomjae

AU - Chun, Hoon-Jai

AU - Lee, Sang Woo

AU - Bak, Young-Tae

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Background and Aim: The aim of this study was to compare HOXB7 expression level between gastric cancer and non-cancerous gastric tissues. Additionally, the functional effects of HOXB7, including its pro-migration or invasion and anti-apoptosis roles, were evaluated in gastric cancer cells. Methods: Both gene and protein expression levels of HOXB7 were examined in gastric cancer cell lines, and HOXB7 expression was compared between primary or metastatic gastric cancer tissues and chronic gastritis or intestinal metaplasia tissues. Functional studies included a wound healing assay, a Matrigel invasion assay, and an Annexin-V assay were performed, and Akt/PTEN activity was measured by western blotting. Results: Both gene and protein expression levels of HOXB7 could be clearly detected in various gastric cancer cell lines except MKN-28 cell. HOXB7 expression was significantly higher in primary or metastatic gastric cancer tissues than in chronic gastritis or intestinal metaplasia tissues. HOXB7 knockdown led to inhibition of cell invasion and migration, had an apoptotic effect, downregulated phosphor-Akt, and upregulated PTEN in AGS and SNU-638 cells. Reinforced expression of HOXB7 caused the opposite effects in MKN-28 and MKN-45 cells. Conclusion: Our study suggests that HOXB7 has an oncogenic role in gastric cancer, which might be related to the modulation of Akt/PTEN activity to induce cell migration/invasion and anti-apoptotic effects.

AB - Background and Aim: The aim of this study was to compare HOXB7 expression level between gastric cancer and non-cancerous gastric tissues. Additionally, the functional effects of HOXB7, including its pro-migration or invasion and anti-apoptosis roles, were evaluated in gastric cancer cells. Methods: Both gene and protein expression levels of HOXB7 were examined in gastric cancer cell lines, and HOXB7 expression was compared between primary or metastatic gastric cancer tissues and chronic gastritis or intestinal metaplasia tissues. Functional studies included a wound healing assay, a Matrigel invasion assay, and an Annexin-V assay were performed, and Akt/PTEN activity was measured by western blotting. Results: Both gene and protein expression levels of HOXB7 could be clearly detected in various gastric cancer cell lines except MKN-28 cell. HOXB7 expression was significantly higher in primary or metastatic gastric cancer tissues than in chronic gastritis or intestinal metaplasia tissues. HOXB7 knockdown led to inhibition of cell invasion and migration, had an apoptotic effect, downregulated phosphor-Akt, and upregulated PTEN in AGS and SNU-638 cells. Reinforced expression of HOXB7 caused the opposite effects in MKN-28 and MKN-45 cells. Conclusion: Our study suggests that HOXB7 has an oncogenic role in gastric cancer, which might be related to the modulation of Akt/PTEN activity to induce cell migration/invasion and anti-apoptotic effects.

KW - Akt

KW - HOXB7

KW - PTEN

KW - stomach cancer

UR - http://www.scopus.com/inward/record.url?scp=85002374778&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85002374778&partnerID=8YFLogxK

U2 - 10.1111/jgh.13330

DO - 10.1111/jgh.13330

M3 - Article

C2 - 26968988

AN - SCOPUS:85002374778

VL - 31

SP - 1717

EP - 1726

JO - Journal of Gastroenterology and Hepatology (Australia)

JF - Journal of Gastroenterology and Hepatology (Australia)

SN - 0815-9319

IS - 10

ER -