The selenoproteome of Clostridium sp. OhILAs: Characterization of anaerobic bacterial selenoprotein methionine sulfoxide reductase A

Hwa Young Kim, Yan Zhang, Byung Cheon Lee, Jae Ryong Kim, Vadim N. Gladyshev

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Selenocysteine (Sec) is incorporated into proteins in response to UGA codons. This residue is frequently found at the catalytic sites of oxidoreductases. In this study, we characterized the selenoproteome of an anaerobic bacterium, Clostridium sp. (also known as Alkaliphilus oremlandii) OhILA, and identified 13 selenoprotein genes, five of which have not been previously described. One of the detected selenoproteins was methionine sulfoxide reductase A (MsrA), an antioxidant enzyme that repairs oxidatively damaged methionines in a stereospecific manner. To date, little is known about MsrA from anaerobes. We characterized this selenoprotein MsrA which had a single Sec residue at the catalytic site but no cysteine (Cys) residues in the protein sequence. Its SECIS (Sec insertion sequence) element did not resemble those in Escherichia coli. Although with low translational efficiency, the expression of the Clostridium selenoprotein msrA gene in E. coli could be demonstrated by 75Se metabolic labeling, immunoblot analyses, and enzyme assays, indicating that its SECIS element was recognized by the E. coli Sec insertion machinery. We found that the Sec-containing MsrA exhibited at least a 20-fold higher activity than its Cys mutant form, indicating a critical role of Sec in the catalytic activity of the enzyme. Furthermore, our data revealed that the Clostridium MsrA was inefficiently reducible by thioredoxin, which is a typical reducing agent for MsrA, suggesting the use of alternative electron donors in this anaerobic bacterium that directly act on the selenenic acid intermediate and do not require resolving Cys residues.

Original languageEnglish
Pages (from-to)1008-1017
Number of pages10
JournalProteins: Structure, Function and Bioinformatics
Volume74
Issue number4
DOIs
Publication statusPublished - 2009 Mar 1
Externally publishedYes

Fingerprint

Methionine Sulfoxide Reductases
Selenoproteins
Selenocysteine
Clostridium
Escherichia coli
Cysteine
DNA Transposable Elements
Anaerobic Bacteria
Catalytic Domain
Bacteria
Enzymes
Genes
Thioredoxins
Terminator Codon
Reducing Agents
Enzyme Assays
Methionine
Labeling
Machinery
Assays

Keywords

  • MsrA
  • Oxidoreductase
  • SECIS elements
  • Selenoproteins
  • Thioredoxin

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

The selenoproteome of Clostridium sp. OhILAs : Characterization of anaerobic bacterial selenoprotein methionine sulfoxide reductase A. / Kim, Hwa Young; Zhang, Yan; Lee, Byung Cheon; Kim, Jae Ryong; Gladyshev, Vadim N.

In: Proteins: Structure, Function and Bioinformatics, Vol. 74, No. 4, 01.03.2009, p. 1008-1017.

Research output: Contribution to journalArticle

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