The sequence variation responsible for the functional difference between the CONSTANS protein, and the CONSTANS-like (COL) 1 and COL2 proteins, resides mostly in the region encoded by their first exons

Soon Kap Kim, Hyo Young Park, Yun Hee Jang, Jeong Hwan Lee, Jeong Kook Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Although the protein CONSTANS (CO) and its close relatives CONSTANS-like (COL) 1 and COL2 exhibit high amino acid sequence similarities, only the CO protein regulates floral induction in Arabidopsis. To investigate the structural basis for the functional differences between CO, COL1, and COL2 in flowering, we performed domain-swapping between CO, COL1, and COL2, and site-directed mutagenesis on the first exon of CO. The results suggest that the lack of flowering promotion activity by COL1 and COL2 is mainly attributed to the differences between CO and the COL1 and COL2 proteins in the amino acid sequence encoded by their first exons.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalPlant Science
Volume199-200
DOIs
Publication statusPublished - 2013 Feb 1

Fingerprint

exons
Exons
Amino Acid Sequence
amino acid sequences
flowering
Proteins
proteins
site-directed mutagenesis
Site-Directed Mutagenesis
Arabidopsis
sequence diversity

Keywords

  • CO
  • COL1
  • COL2
  • Domain swapping
  • Evolution
  • Flowering time

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Agronomy and Crop Science

Cite this

The sequence variation responsible for the functional difference between the CONSTANS protein, and the CONSTANS-like (COL) 1 and COL2 proteins, resides mostly in the region encoded by their first exons. / Kim, Soon Kap; Park, Hyo Young; Jang, Yun Hee; Lee, Jeong Hwan; Kim, Jeong Kook.

In: Plant Science, Vol. 199-200, 01.02.2013, p. 71-78.

Research output: Contribution to journalArticle

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