The SH2-SH2-SH3 domain of phospholipase C-γ1 directly binds to translational elongation factor-1α

Myung Jong Kim, Fuchum Si, Su Jeong Kim, Seung Bum Hong, Jong-Ik Hwang, He Jin Lee, Seung Jae Lee, Jong Soo Chang, Young Han Lee, Sung Ho Ryu, Pann Ghill Suh

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Phospholipase C-γ1 (PLC-γ1) is a lipase that hydrolyzes PIP2 to generate two second messengers, IP3 and DAG. By using the yeast two-hybrid system, we identified the translational elongation factor-1α (EF-1α) as a binding protein of PLC-γ1 from the human B-lymphocyte library. Direct interaction between EF-1α and PLC-γ1 was confirmed by the in vitro binding experiment using purified PLC-γ1. Furthermore, from the in vitro binding experiment, we could demonstrate that the carboxyl terminal region of EF-1α is involved in the interaction with PLC-γ1, and that both SH2 and SH3 domains of PLC-γ1 are required for the interaction with EF-1α. In vivo interaction between EF-1α and PLC-γ1 was confirmed by the immunoprecipitation experiment using anti-EF-1α antibody. The interaction between EF-1α and PLC-γ1 was enhanced by EGF-treatment. Taken together, we suggest that EF-1α might play a role in PLC-γ1-mediated signal transduction.

Original languageEnglish
Pages (from-to)631-637
Number of pages7
JournalMolecules and Cells
Volume9
Issue number6
Publication statusPublished - 1999 Dec 31
Externally publishedYes

Fingerprint

Peptide Elongation Factor 1
src Homology Domains
Type C Phospholipases
Two-Hybrid System Techniques
Second Messenger Systems
Protein C
Lipase
Immunoprecipitation
Epidermal Growth Factor
Signal Transduction
Carrier Proteins
B-Lymphocytes

Keywords

  • EF-1α
  • PLC-γ1
  • Src Homology Domain
  • Yeast Two Hybrid System

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Kim, M. J., Si, F., Kim, S. J., Hong, S. B., Hwang, J-I., Lee, H. J., ... Suh, P. G. (1999). The SH2-SH2-SH3 domain of phospholipase C-γ1 directly binds to translational elongation factor-1α. Molecules and Cells, 9(6), 631-637.

The SH2-SH2-SH3 domain of phospholipase C-γ1 directly binds to translational elongation factor-1α. / Kim, Myung Jong; Si, Fuchum; Kim, Su Jeong; Hong, Seung Bum; Hwang, Jong-Ik; Lee, He Jin; Lee, Seung Jae; Chang, Jong Soo; Lee, Young Han; Ryu, Sung Ho; Suh, Pann Ghill.

In: Molecules and Cells, Vol. 9, No. 6, 31.12.1999, p. 631-637.

Research output: Contribution to journalArticle

Kim, MJ, Si, F, Kim, SJ, Hong, SB, Hwang, J-I, Lee, HJ, Lee, SJ, Chang, JS, Lee, YH, Ryu, SH & Suh, PG 1999, 'The SH2-SH2-SH3 domain of phospholipase C-γ1 directly binds to translational elongation factor-1α', Molecules and Cells, vol. 9, no. 6, pp. 631-637.
Kim, Myung Jong ; Si, Fuchum ; Kim, Su Jeong ; Hong, Seung Bum ; Hwang, Jong-Ik ; Lee, He Jin ; Lee, Seung Jae ; Chang, Jong Soo ; Lee, Young Han ; Ryu, Sung Ho ; Suh, Pann Ghill. / The SH2-SH2-SH3 domain of phospholipase C-γ1 directly binds to translational elongation factor-1α. In: Molecules and Cells. 1999 ; Vol. 9, No. 6. pp. 631-637.
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