The significance of hydrated surface molecular mobility in the control of the morphology of adhering fibroblasts

Ji-Hun Seo, Sachiro Kakinoki, Yuuki Inoue, Kwangwoo Nam, Tetsuji Yamaoka, Kazuhiko Ishihara, Akio Kishida, Nobuhiko Yui

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The effects of the hydrated molecular mobility and the surface free energy of polymer surfaces on fibronectin adsorption and fibroblast adhesion were investigated. ABA-type block copolymers composed of polyrotaxane (PRX) with different number of threaded α-cyclodextrin (α-CD), random copolymers with similar chemical composition to the PRX block copolymers, and conventional polymers were prepared to determine a wide range of hydrated molecular mobility (Mf) values estimated by quartz crystal microbalance-dissipation (QCM-D) measurements. Fibronectin adsorption was highly dependent on surface free energy, and high surface fibronectin density resulted in a large projected cell area on the polymer surfaces. However, the morphology of adhering fibroblasts was not explained by the surface free energy, but it was found to be strongly dependent on the Mf values of the polymer surfaces in aqueous media. These results emphasize the importance of Mf in the discussion of the elongated morphology of adhering fibroblasts on various polymer surfaces.

Original languageEnglish
Pages (from-to)3206-3214
Number of pages9
JournalBiomaterials
Volume34
Issue number13
DOIs
Publication statusPublished - 2013 Apr 1
Externally publishedYes

Fingerprint

Fibroblasts
Polymers
Fibronectins
Adsorption
Free energy
Quartz Crystal Microbalance Techniques
Block copolymers
Cyclodextrins
Quartz crystal microbalances
Adhesion
Copolymers
Chemical analysis
polyrotaxane

Keywords

  • Cell adhesion
  • Molecular mobility
  • Polyrotaxane
  • Protein adsorption
  • Surface free energy

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

The significance of hydrated surface molecular mobility in the control of the morphology of adhering fibroblasts. / Seo, Ji-Hun; Kakinoki, Sachiro; Inoue, Yuuki; Nam, Kwangwoo; Yamaoka, Tetsuji; Ishihara, Kazuhiko; Kishida, Akio; Yui, Nobuhiko.

In: Biomaterials, Vol. 34, No. 13, 01.04.2013, p. 3206-3214.

Research output: Contribution to journalArticle

Seo, J-H, Kakinoki, S, Inoue, Y, Nam, K, Yamaoka, T, Ishihara, K, Kishida, A & Yui, N 2013, 'The significance of hydrated surface molecular mobility in the control of the morphology of adhering fibroblasts', Biomaterials, vol. 34, no. 13, pp. 3206-3214. https://doi.org/10.1016/j.biomaterials.2013.01.080
Seo, Ji-Hun ; Kakinoki, Sachiro ; Inoue, Yuuki ; Nam, Kwangwoo ; Yamaoka, Tetsuji ; Ishihara, Kazuhiko ; Kishida, Akio ; Yui, Nobuhiko. / The significance of hydrated surface molecular mobility in the control of the morphology of adhering fibroblasts. In: Biomaterials. 2013 ; Vol. 34, No. 13. pp. 3206-3214.
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