The Simple Determination Method for Anthocyanidin Aglycones in Fruits Using Ultra-High-Performance Liquid Chromatography

You Shin Shim, Won Jin Yoon, Dong Man Kim, Masaki Watanabe, Hyun Jin Park, Hae Won Jang, Jangho Lee, Jaeho Ha

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The simple determination method for anthocyanidin aglycones in fruits using ultra-high-performance liquid chromatography (UHPLC) coupled with the heating-block acidic hydrolysis method was validated through the precision, accuracy and linearity. The UHPLC separation was performed on a reversed-phase C18 column (particle size 2 μm, i.d. 2 mm, length 100 mm) with a photodiode-array detector. The limits of detection and quantification of the UHPLC analyses were 0.09 and 0.29 mg/kg for delphinidin, 0.08 and 0.24 mg/kg for cyanidin, 0.09 and 0.26 mg/kg for petunidin, 0.14 and 0.42 mg/kg for pelargonidin, 0.16 and 0.48 mg/kg for peonidin and 0.30 and 0.91 mg/kg for malvidin, respectively. The intra- and inter-day precisions of individual anthocyanidin aglycones were

Original languageEnglish
Pages (from-to)1646-1653
Number of pages8
JournalJournal of Chromatographic Science
Issue number10
Publication statusPublished - 2015 Nov 1


ASJC Scopus subject areas

  • Analytical Chemistry

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