The Simple Determination Method for Anthocyanidin Aglycones in Fruits Using Ultra-High-Performance Liquid Chromatography

You Shin Shim, Won Jin Yoon, Dong Man Kim, Masaki Watanabe, Hyun Jin Park, Hae Won Jang, Jangho Lee, Jaeho Ha

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The simple determination method for anthocyanidin aglycones in fruits using ultra-high-performance liquid chromatography (UHPLC) coupled with the heating-block acidic hydrolysis method was validated through the precision, accuracy and linearity. The UHPLC separation was performed on a reversed-phase C18 column (particle size 2 μm, i.d. 2 mm, length 100 mm) with a photodiode-array detector. The limits of detection and quantification of the UHPLC analyses were 0.09 and 0.29 mg/kg for delphinidin, 0.08 and 0.24 mg/kg for cyanidin, 0.09 and 0.26 mg/kg for petunidin, 0.14 and 0.42 mg/kg for pelargonidin, 0.16 and 0.48 mg/kg for peonidin and 0.30 and 0.91 mg/kg for malvidin, respectively. The intra- and inter-day precisions of individual anthocyanidin aglycones were <10.3%. All calibration curves exhibited good linearity (r = 0.999) within the tested ranges. The total run time of UHPLC was 8 min. The simple preparation method with UHPLC detection in this study presented herein significantly improved the speed and the simplicity for preparation step of delphinidin, cyanidin, petunidin, pelargonidin, peonidin and malvidin in fruits. Especially, the UHPLC detection exhibited good resolution in spite of shorter run time about four times than conventional HPLC detection.

Original languageEnglish
Pages (from-to)1646-1653
Number of pages8
JournalJournal of Chromatographic Science
Volume53
Issue number10
DOIs
Publication statusPublished - 2015 Nov

ASJC Scopus subject areas

  • Analytical Chemistry

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