The strategy of signal amplification for ultrasensitive detection of hIgE based on aptamer-modified poly(di-acetylene) supramolecules

Jun Pyo Kim, Il Kyoung Kwon, Sang Jun Sim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Herein, we demonstrate three strategies of signal amplification for ultrasensitive detection of human immunoglobulin E (hIgE) based on poly(di-acetylene) supramolecules. To fabricate the ultrasensitive PDA biosensor, ethylenediamine as an interlinker and aptamer as a receptor were introduced into the chip fabrication process. Using the prepared PDA liposome biosensor, the hIgE could be detected up to below 1.0. ng/ml by a primary response. In order to accomplish more ultrasensitive detection of protein on a PDA biosensor, polyclonal hIgE antibody was employed as an external mechanical force for the inducement of a secondary response. As a result, a PDA liposome biosensor sensitivity as high as 0.01. ng/ml for the target hIgE was obtained, with a sensitivity which is one hundred times of that of the method without signal amplification. These results indicate that the proposed strategies were capable of ultrasensitive quantitative and qualitative analyses of biomolecules without non-specific binding of non-target proteins.

Original languageEnglish
Pages (from-to)4823-4827
Number of pages5
JournalBiosensors and Bioelectronics
Volume26
Issue number12
DOIs
Publication statusPublished - 2011 Aug 15

Keywords

  • Allergy diagnosis
  • Aptamer
  • Fluorescence biosensor
  • Interlinker
  • Polydiacetylene
  • Signal amplification

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

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