The structures of HslU and the ATP-dependent protease HslU-HslV

Matthias Bochtler, Claudia Hartmann, Hyun Kyu Song, Gleb P. Bourenkov, Hans D. Bartunik, Robert Huber

Research output: Contribution to journalArticle

363 Citations (Scopus)

Abstract

The degradation of cytoplasmic proteins is an ATP-dependent process. Substrates are targeted to a single soluble protease, the 26S proteasome, in eukaryotes and to a number of unrelated proteases in prokaryotes. A surprising link emerged with the discovery of the ATP-dependent protease HslVU (heat shock locus VU) in Escherichia coli. Its protease component HslV shares ~20% sequence similarity and a conserved fold with 20S proteasome β- subunits. HslU is a member of the Hsp100 (Clp) family of ATPases. Here we report the crystal structures of free HslU and an 820,000 relative molecular mass complex of HslU and HslV-the first structure of a complete set of components of an ATP-dependent protease. HslV and HslU display sixfold symmetry, ruling out mechanisms of protease activation that require a symmetry mismatch between the two components. Instead, there is conformational flexibility and domain motion in HslU and a localized order- disorder transition in HslV. Individual subunits of HslU contain two globular domains in relative orientations that correlate with nucleotide bound and unbound states. They are surprisingly similar to their counterparts in N- ethylmaleimide-sensitive fusion protein, the prototype of an AAA-ATPase. A third, mostly α-helical domain in HslU mediates the contact with HslV and may be the structural equivalent of the amino-terminal domains in proteasomal AAA-ATPases.

Original languageEnglish
Pages (from-to)800-805
Number of pages6
JournalNature
Volume403
Issue number6771
DOIs
Publication statusPublished - 2000 Feb 17
Externally publishedYes

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ATP-Dependent Proteases
Peptide Hydrolases
Adenosine Triphosphatases
N-Ethylmaleimide-Sensitive Proteins
Proteasome Endopeptidase Complex
Eukaryota
Proteolysis
Shock
Nucleotides
Hot Temperature
Adenosine Triphosphate
Escherichia coli

ASJC Scopus subject areas

  • General

Cite this

Bochtler, M., Hartmann, C., Song, H. K., Bourenkov, G. P., Bartunik, H. D., & Huber, R. (2000). The structures of HslU and the ATP-dependent protease HslU-HslV. Nature, 403(6771), 800-805. https://doi.org/10.1038/35001629

The structures of HslU and the ATP-dependent protease HslU-HslV. / Bochtler, Matthias; Hartmann, Claudia; Song, Hyun Kyu; Bourenkov, Gleb P.; Bartunik, Hans D.; Huber, Robert.

In: Nature, Vol. 403, No. 6771, 17.02.2000, p. 800-805.

Research output: Contribution to journalArticle

Bochtler, M, Hartmann, C, Song, HK, Bourenkov, GP, Bartunik, HD & Huber, R 2000, 'The structures of HslU and the ATP-dependent protease HslU-HslV', Nature, vol. 403, no. 6771, pp. 800-805. https://doi.org/10.1038/35001629
Bochtler M, Hartmann C, Song HK, Bourenkov GP, Bartunik HD, Huber R. The structures of HslU and the ATP-dependent protease HslU-HslV. Nature. 2000 Feb 17;403(6771):800-805. https://doi.org/10.1038/35001629
Bochtler, Matthias ; Hartmann, Claudia ; Song, Hyun Kyu ; Bourenkov, Gleb P. ; Bartunik, Hans D. ; Huber, Robert. / The structures of HslU and the ATP-dependent protease HslU-HslV. In: Nature. 2000 ; Vol. 403, No. 6771. pp. 800-805.
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