The suppressive effect of evening primrose oil on murine contact sensitivity

J. H. Hong, S. Y. Lee, Hae Jun Song, Young Chul Kye, S. N. Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Evening primrose oil (EPO) is a rich source of cis-linoleic acid and gammalinolenic acid (GLA) and has been used as a therapeutic agent in various skin diseases such as atopic dermatitis. Objective: The purpose of this study was to evaluate the suppressive effect of EPO on murine contact sensitivity. Methods: BALB/c mice were divided into 3 groups, positive control, experimental and negative control groups: the positive control group represents a group of mice which were sensitized and challenged with DNFB, the experimental group represents EPO-pretreated positive control group and the negative control group represents a group of mice which were challenged only. The changes of ear thickness were measured, and H and E staining and immunohistochemical staining for ICAM-1 expression of ear skin were performed to evaluate the histological changes of each group. Results: The pretreatment of mice with EPO resulted in suppression of contact sensitivity by more than 82%. On H and E staining, only a mild inflammatory reaction was observed in the dermis. Also ICAM-1 expression of keratinocytes, the intensity of the staining was significantly decreased in the experimental group compared with positive group. Conclusions: Our study indicates that EPO was able to suppress the induction of contact sensitivity.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalAnnals of Dermatology
Volume7
Issue number1
Publication statusPublished - 1995 Jan 1

Fingerprint

Contact Dermatitis
Control Groups
Staining and Labeling
Intercellular Adhesion Molecule-1
Ear
Dinitrofluorobenzene
Linoleic Acid
Atopic Dermatitis
Dermis
Keratinocytes
Skin Diseases
Efamol
Skin
Acids

Keywords

  • contract sensitivity
  • essential fatty acid (EFA)
  • evening primrose oil (EPO)

ASJC Scopus subject areas

  • Dermatology

Cite this

The suppressive effect of evening primrose oil on murine contact sensitivity. / Hong, J. H.; Lee, S. Y.; Song, Hae Jun; Kye, Young Chul; Kim, S. N.

In: Annals of Dermatology, Vol. 7, No. 1, 01.01.1995, p. 39-44.

Research output: Contribution to journalArticle

@article{0f5dcc3c92b54c40a70ac9a2f8d6dcb2,
title = "The suppressive effect of evening primrose oil on murine contact sensitivity",
abstract = "Background: Evening primrose oil (EPO) is a rich source of cis-linoleic acid and gammalinolenic acid (GLA) and has been used as a therapeutic agent in various skin diseases such as atopic dermatitis. Objective: The purpose of this study was to evaluate the suppressive effect of EPO on murine contact sensitivity. Methods: BALB/c mice were divided into 3 groups, positive control, experimental and negative control groups: the positive control group represents a group of mice which were sensitized and challenged with DNFB, the experimental group represents EPO-pretreated positive control group and the negative control group represents a group of mice which were challenged only. The changes of ear thickness were measured, and H and E staining and immunohistochemical staining for ICAM-1 expression of ear skin were performed to evaluate the histological changes of each group. Results: The pretreatment of mice with EPO resulted in suppression of contact sensitivity by more than 82{\%}. On H and E staining, only a mild inflammatory reaction was observed in the dermis. Also ICAM-1 expression of keratinocytes, the intensity of the staining was significantly decreased in the experimental group compared with positive group. Conclusions: Our study indicates that EPO was able to suppress the induction of contact sensitivity.",
keywords = "contract sensitivity, essential fatty acid (EFA), evening primrose oil (EPO)",
author = "Hong, {J. H.} and Lee, {S. Y.} and Song, {Hae Jun} and Kye, {Young Chul} and Kim, {S. N.}",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "7",
pages = "39--44",
journal = "Annals of Dermatology",
issn = "1013-9087",
publisher = "Korean Dermatological Association",
number = "1",

}

TY - JOUR

T1 - The suppressive effect of evening primrose oil on murine contact sensitivity

AU - Hong, J. H.

AU - Lee, S. Y.

AU - Song, Hae Jun

AU - Kye, Young Chul

AU - Kim, S. N.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Background: Evening primrose oil (EPO) is a rich source of cis-linoleic acid and gammalinolenic acid (GLA) and has been used as a therapeutic agent in various skin diseases such as atopic dermatitis. Objective: The purpose of this study was to evaluate the suppressive effect of EPO on murine contact sensitivity. Methods: BALB/c mice were divided into 3 groups, positive control, experimental and negative control groups: the positive control group represents a group of mice which were sensitized and challenged with DNFB, the experimental group represents EPO-pretreated positive control group and the negative control group represents a group of mice which were challenged only. The changes of ear thickness were measured, and H and E staining and immunohistochemical staining for ICAM-1 expression of ear skin were performed to evaluate the histological changes of each group. Results: The pretreatment of mice with EPO resulted in suppression of contact sensitivity by more than 82%. On H and E staining, only a mild inflammatory reaction was observed in the dermis. Also ICAM-1 expression of keratinocytes, the intensity of the staining was significantly decreased in the experimental group compared with positive group. Conclusions: Our study indicates that EPO was able to suppress the induction of contact sensitivity.

AB - Background: Evening primrose oil (EPO) is a rich source of cis-linoleic acid and gammalinolenic acid (GLA) and has been used as a therapeutic agent in various skin diseases such as atopic dermatitis. Objective: The purpose of this study was to evaluate the suppressive effect of EPO on murine contact sensitivity. Methods: BALB/c mice were divided into 3 groups, positive control, experimental and negative control groups: the positive control group represents a group of mice which were sensitized and challenged with DNFB, the experimental group represents EPO-pretreated positive control group and the negative control group represents a group of mice which were challenged only. The changes of ear thickness were measured, and H and E staining and immunohistochemical staining for ICAM-1 expression of ear skin were performed to evaluate the histological changes of each group. Results: The pretreatment of mice with EPO resulted in suppression of contact sensitivity by more than 82%. On H and E staining, only a mild inflammatory reaction was observed in the dermis. Also ICAM-1 expression of keratinocytes, the intensity of the staining was significantly decreased in the experimental group compared with positive group. Conclusions: Our study indicates that EPO was able to suppress the induction of contact sensitivity.

KW - contract sensitivity

KW - essential fatty acid (EFA)

KW - evening primrose oil (EPO)

UR - http://www.scopus.com/inward/record.url?scp=0029052073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029052073&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0029052073

VL - 7

SP - 39

EP - 44

JO - Annals of Dermatology

JF - Annals of Dermatology

SN - 1013-9087

IS - 1

ER -