The synergic anti-inflammatory impact of Gleditsia sinensis Lam. and Lactobacillus brevis KY21 on intestinal epithelial cells in a DSS-induced colitis model

Younghoon Kim, Ji Hoon Koh, Young Jun Ahn, Sejong Oh, Sea Hun Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We investigated the synergic anti-inflammatory activity of Gleditsia sinensis Lam. (GS) extract and Lactobacillus brevis KY21 both in vitro and in vivo. Western blot analysis and immunostaining showed that AKT phosphorylation that increased by the exposure of LPS were significantly decreased by the presence of either GS extract or L. brevis KY21. In addition, p65 intracellular transport was critically inhibited by GS extract and L. brevis KY21. We further studied these effects using an in vivo dextran sulfate sodium (DSS)-induced mouse model. Body weight, food intake, and clinical scores were dramatically decreased after treatment with DSS, whereas these effects were palliated by the addition of GS extract and L. brevis KY21. Importantly, transcription of genes encoding pro-inflammatory cytokines including IL-1β, TNF-α, and IFN-γ in mesenteric lymph nodes (MLN) and the spleen were increased by DSS treatment, whereas they were inhibited by the presence of GS extract and L. brevis KY21.

Original languageEnglish
Pages (from-to)604-610
Number of pages7
JournalKorean Journal for Food Science of Animal Resources
Volume35
Issue number5
DOIs
Publication statusPublished - 2015 Jan 1

Keywords

  • Anti-inflammatory activity
  • DSS
  • Herbal extract
  • Probiotic bacteria

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology

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