The TGF-β-induced gene product, βig-h3: Its biological implications in peritoneal dialysis

Sun Hee Park, Soon Youn Choi, Mi Hyung Kim, Eun Joo Oh, Hye Myung Ryu, Chan Duck Kim, In San Kim, Yong Lim Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background. TGF-β is involved in peritoneal changes during long-term peritoneal dialysis (PD). TGF-β induces βig-h3 in several cell lines, and βig-h3 may be a marker for biologically active TGF-β. However, no study has reported induction of βig-h3 in human peritoneal mesothelial cells (HPMCs) or its involvement in PD-related peritoneal membrane changes. Methods. We used cultured HPMCs to investigate the biological roles of βig-h3 during mesothelial cell injury and repair, employing the adhesion, spreading, scratching and cell migration assays. Changes in βig-h3 expression after high glucose exposure in vivo were also evaluated using an animal chronic PD model. Results. In vitro, TGF-β1 induced βig-h3 in cultured HPMCs, and βig-h3-mediated mesothelial cell adhesion occurred via αvβ3 integrin. βig-h3 enhanced mesothelial cell adhesion and migration and, in part, wound healing during mesothelial cell injury. The animal study demonstrated that compared to the control group, βig-h3 concentrations in the dialysate effluent increased in the dialysis group with alterations in peritoneal structure and function during PD, and βig-h3 positively correlated with peritoneal solute transport. Immunohistochemical and immunoblotting results showed that βig-h3 localizes in the mesothelium and submesothelial matrix of the parietal peritoneum, and in the vascular endothelium of omentum. βig-h3 protein expression was higher in the dialysis group. Conclusion. In vitro, βig-h3 induced by TGF-β1 in HPMCs improved adhesion and migration of HPMCs during wound healing. In the chronic infusion model of PD, βig-h3 played a role in the functional deterioration of the peritoneal membrane, which is associated with fibrosis.

Original languageEnglish
Pages (from-to)126-135
Number of pages10
JournalNephrology Dialysis Transplantation
Volume23
Issue number1
DOIs
Publication statusPublished - 2008 Jan
Externally publishedYes

Keywords

  • Fibrosis
  • High glucose
  • Mesothelial cells
  • Peritoneum
  • TGF-β1
  • βig-h3

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

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