@article{420b63aa805547fb81353519ffab2717,
title = "The transcription factor γMYB2 acts as a negative regulator of secondary cell wall thickening in anther and stem",
abstract = " Secondary cell wall (SCW) thickening provides the mechanical force for anther dehiscence and plays an important role in the formation of xylem structure. We have previously reported that γMYB2, a MYB coiled-coil protein, directly binds to the P1BS cis-element of the PLA 2 -γ promoter and acts as a co-activator of γMYB1 in controlling the expression of PLA 2 -γ. In this study, we analyzed morphological phenotypes of the constitutive overexpression (γMYB2-OE) and knock-down (γMYB2-KD) lines of γMYB2. We found that γMYB2 overexpression caused the collapse of the endothecium layer, thereby suppressing anther dehiscence and forming short infertile siliques. The γMYB2-OE also showed less cellulose deposition in the xylem and had a longer primary stem than the wild-type, while γMYB2-KD had greater cellulose accumulation and a shorter primary stem than the wild-type. We demonstrated that the male sterility and the longer primary stem in γMYB2-OE were caused by reduced expression of SCW thickening-related genes. Our results suggest that γMYB2 acts as a negative regulator in controlling the SCW thickening in Arabidopsis. ",
keywords = "Anther dehiscence, Arabidopsis thaliana, Endothecium, Negative regulator, Secondary cell wall, γMYB2",
author = "Nguyen, {Ha Thi Kim} and Sujin Hyoung and Kim, {Hae Jin} and Cho, {Kwang Moon} and Shin, {Jeong Sheop}",
note = "Funding Information: We would like to thank to Dr. Mitsuda (Bioproduction Research Institute, The National Institute of Advanced Industrial Science and Technology, JAPAN) for providing seeds of double knock-down mutant nst1nst2. This work was supported by a grant (NRF-2014R1A2A2A01005974) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology and by a grant (PJ013670) from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration, Republic of Korea. This work was also partially supported by Korea University. Funding Information: We would like to thank to Dr. Mitsuda (Bioproduction Research Institute, The National Institute of Advanced Industrial Science and Technology, JAPAN) for providing seeds of double knock-down mutant nst1nst2. This work was supported by a grant ( NRF-2014R1A2A2A01005974 ) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology and by a grant ( PJ013670 ) from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration , Republic of Korea. This work was also partially supported by Korea University . ",
year = "2019",
month = jun,
day = "20",
doi = "10.1016/j.gene.2019.03.061",
language = "English",
volume = "702",
pages = "158--165",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
}