The transcriptional co-activator TAZ interacts differentially with transcriptional enhancer factor-1 (TEF-1) family members

William M. Mahoney, Jeong-Ho Hong, Michael B. Yaffe, Iain K G Farrance

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Members of the highly related TEF-1 (transcriptional enhancer factor-1) family (also known as TEAD, for TEF-1, TEC1, ABAA domain) bind to MCAT (muscle C, A and T sites) and A/T-rich sites in promoters active in cardiac, skeletal and smooth muscle, placenta, and neural crest. TEF-1 activity is regulated by interactions with transcriptional co-factors [p160, TONDU (Vgl-1, Vestigial-like protein-1), Vgl-2 and YAP65 (Yes-associated protein 63 kDa)]. The strong transcriptional co-activator YAP65 interacts with all TEF-1 family members, and, since YAP65 is related to TAZ (transcriptional co-activator with PDZ-binding motif), we wanted to determine if TAZ also interacts with members of the TEF-1 family. In the present study, we show by GST (glutathione S-transferase) pull-down assays, by co-immunoprecipitation and by modified mammalian two-hybrid assays that TEF-1 interacts with TAZ in vitro and in vivo. Electrophoretic mobility-shift assays with purified TEF-1 and GST-TAZ fusion protein showed that TAZ interacts with TEF-1 bound to MCAT DNA, TAZ can interact with endogenous TEF-1 proteins, since exogenous TAZ activated MCAT-dependent reporter promoters. Like YAP65, TAZ interacted with all four TEF-1 family members. GST pull-down assays with increasing amounts of [35S]TEF-1 and [ 35S]RTEF-1 (related TEF-1) showed that TAZ interacts more efficiently with TEF-1 than with RTEF-1. This differential interaction also extended to the interaction of TEF-1 and RTEF-1 with TAZ in vivo, as assayed by a modified mammalian two-hybrid experiment. These data show that differential association of TEF-1 proteins with transcriptional co-activators may regulate the activity of TEF-1 family members.

Original languageEnglish
Pages (from-to)217-225
Number of pages9
JournalBiochemical Journal
Volume388
Issue number1
DOIs
Publication statusPublished - 2005 May 15
Externally publishedYes

Fingerprint

Muscle
Assays
Glutathione Transferase
Proteins
Muscles
Electrophoretic mobility
Two-Hybrid System Techniques
Neural Crest
Electrophoretic Mobility Shift Assay
Immunoprecipitation
Placenta
Smooth Muscle
Myocardium
Skeletal Muscle
Fusion reactions
Association reactions
DNA
Experiments

Keywords

  • ABAA domain (TEAD)
  • Muscle
  • TEC1
  • TEF-1
  • Transcriptional co-activator
  • Transcriptional co-activator with PDZ-binding motif (TAZ)
  • Transcriptional enhancer factor-1 (TEF-1)
  • Yes-associated protein 65 kDa (YAP65)

ASJC Scopus subject areas

  • Biochemistry

Cite this

The transcriptional co-activator TAZ interacts differentially with transcriptional enhancer factor-1 (TEF-1) family members. / Mahoney, William M.; Hong, Jeong-Ho; Yaffe, Michael B.; Farrance, Iain K G.

In: Biochemical Journal, Vol. 388, No. 1, 15.05.2005, p. 217-225.

Research output: Contribution to journalArticle

Mahoney, William M. ; Hong, Jeong-Ho ; Yaffe, Michael B. ; Farrance, Iain K G. / The transcriptional co-activator TAZ interacts differentially with transcriptional enhancer factor-1 (TEF-1) family members. In: Biochemical Journal. 2005 ; Vol. 388, No. 1. pp. 217-225.
@article{0220b471ef244b22b803d6044c1a234d,
title = "The transcriptional co-activator TAZ interacts differentially with transcriptional enhancer factor-1 (TEF-1) family members",
abstract = "Members of the highly related TEF-1 (transcriptional enhancer factor-1) family (also known as TEAD, for TEF-1, TEC1, ABAA domain) bind to MCAT (muscle C, A and T sites) and A/T-rich sites in promoters active in cardiac, skeletal and smooth muscle, placenta, and neural crest. TEF-1 activity is regulated by interactions with transcriptional co-factors [p160, TONDU (Vgl-1, Vestigial-like protein-1), Vgl-2 and YAP65 (Yes-associated protein 63 kDa)]. The strong transcriptional co-activator YAP65 interacts with all TEF-1 family members, and, since YAP65 is related to TAZ (transcriptional co-activator with PDZ-binding motif), we wanted to determine if TAZ also interacts with members of the TEF-1 family. In the present study, we show by GST (glutathione S-transferase) pull-down assays, by co-immunoprecipitation and by modified mammalian two-hybrid assays that TEF-1 interacts with TAZ in vitro and in vivo. Electrophoretic mobility-shift assays with purified TEF-1 and GST-TAZ fusion protein showed that TAZ interacts with TEF-1 bound to MCAT DNA, TAZ can interact with endogenous TEF-1 proteins, since exogenous TAZ activated MCAT-dependent reporter promoters. Like YAP65, TAZ interacted with all four TEF-1 family members. GST pull-down assays with increasing amounts of [35S]TEF-1 and [ 35S]RTEF-1 (related TEF-1) showed that TAZ interacts more efficiently with TEF-1 than with RTEF-1. This differential interaction also extended to the interaction of TEF-1 and RTEF-1 with TAZ in vivo, as assayed by a modified mammalian two-hybrid experiment. These data show that differential association of TEF-1 proteins with transcriptional co-activators may regulate the activity of TEF-1 family members.",
keywords = "ABAA domain (TEAD), Muscle, TEC1, TEF-1, Transcriptional co-activator, Transcriptional co-activator with PDZ-binding motif (TAZ), Transcriptional enhancer factor-1 (TEF-1), Yes-associated protein 65 kDa (YAP65)",
author = "Mahoney, {William M.} and Jeong-Ho Hong and Yaffe, {Michael B.} and Farrance, {Iain K G}",
year = "2005",
month = "5",
day = "15",
doi = "10.1042/BJ20041434",
language = "English",
volume = "388",
pages = "217--225",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "1",

}

TY - JOUR

T1 - The transcriptional co-activator TAZ interacts differentially with transcriptional enhancer factor-1 (TEF-1) family members

AU - Mahoney, William M.

AU - Hong, Jeong-Ho

AU - Yaffe, Michael B.

AU - Farrance, Iain K G

PY - 2005/5/15

Y1 - 2005/5/15

N2 - Members of the highly related TEF-1 (transcriptional enhancer factor-1) family (also known as TEAD, for TEF-1, TEC1, ABAA domain) bind to MCAT (muscle C, A and T sites) and A/T-rich sites in promoters active in cardiac, skeletal and smooth muscle, placenta, and neural crest. TEF-1 activity is regulated by interactions with transcriptional co-factors [p160, TONDU (Vgl-1, Vestigial-like protein-1), Vgl-2 and YAP65 (Yes-associated protein 63 kDa)]. The strong transcriptional co-activator YAP65 interacts with all TEF-1 family members, and, since YAP65 is related to TAZ (transcriptional co-activator with PDZ-binding motif), we wanted to determine if TAZ also interacts with members of the TEF-1 family. In the present study, we show by GST (glutathione S-transferase) pull-down assays, by co-immunoprecipitation and by modified mammalian two-hybrid assays that TEF-1 interacts with TAZ in vitro and in vivo. Electrophoretic mobility-shift assays with purified TEF-1 and GST-TAZ fusion protein showed that TAZ interacts with TEF-1 bound to MCAT DNA, TAZ can interact with endogenous TEF-1 proteins, since exogenous TAZ activated MCAT-dependent reporter promoters. Like YAP65, TAZ interacted with all four TEF-1 family members. GST pull-down assays with increasing amounts of [35S]TEF-1 and [ 35S]RTEF-1 (related TEF-1) showed that TAZ interacts more efficiently with TEF-1 than with RTEF-1. This differential interaction also extended to the interaction of TEF-1 and RTEF-1 with TAZ in vivo, as assayed by a modified mammalian two-hybrid experiment. These data show that differential association of TEF-1 proteins with transcriptional co-activators may regulate the activity of TEF-1 family members.

AB - Members of the highly related TEF-1 (transcriptional enhancer factor-1) family (also known as TEAD, for TEF-1, TEC1, ABAA domain) bind to MCAT (muscle C, A and T sites) and A/T-rich sites in promoters active in cardiac, skeletal and smooth muscle, placenta, and neural crest. TEF-1 activity is regulated by interactions with transcriptional co-factors [p160, TONDU (Vgl-1, Vestigial-like protein-1), Vgl-2 and YAP65 (Yes-associated protein 63 kDa)]. The strong transcriptional co-activator YAP65 interacts with all TEF-1 family members, and, since YAP65 is related to TAZ (transcriptional co-activator with PDZ-binding motif), we wanted to determine if TAZ also interacts with members of the TEF-1 family. In the present study, we show by GST (glutathione S-transferase) pull-down assays, by co-immunoprecipitation and by modified mammalian two-hybrid assays that TEF-1 interacts with TAZ in vitro and in vivo. Electrophoretic mobility-shift assays with purified TEF-1 and GST-TAZ fusion protein showed that TAZ interacts with TEF-1 bound to MCAT DNA, TAZ can interact with endogenous TEF-1 proteins, since exogenous TAZ activated MCAT-dependent reporter promoters. Like YAP65, TAZ interacted with all four TEF-1 family members. GST pull-down assays with increasing amounts of [35S]TEF-1 and [ 35S]RTEF-1 (related TEF-1) showed that TAZ interacts more efficiently with TEF-1 than with RTEF-1. This differential interaction also extended to the interaction of TEF-1 and RTEF-1 with TAZ in vivo, as assayed by a modified mammalian two-hybrid experiment. These data show that differential association of TEF-1 proteins with transcriptional co-activators may regulate the activity of TEF-1 family members.

KW - ABAA domain (TEAD)

KW - Muscle

KW - TEC1

KW - TEF-1

KW - Transcriptional co-activator

KW - Transcriptional co-activator with PDZ-binding motif (TAZ)

KW - Transcriptional enhancer factor-1 (TEF-1)

KW - Yes-associated protein 65 kDa (YAP65)

UR - http://www.scopus.com/inward/record.url?scp=19544393341&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19544393341&partnerID=8YFLogxK

U2 - 10.1042/BJ20041434

DO - 10.1042/BJ20041434

M3 - Article

VL - 388

SP - 217

EP - 225

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 1

ER -