TY - JOUR
T1 - The type III calcium/calmodulin-sensitive adenylyl cyclase is not specific to olfactory sensory neurons
AU - Xia, Zhengui
AU - Choi, Eui Ju
AU - Wang, Fan
AU - Storm, Daniel R.
N1 - Funding Information:
We would like to thank Dr R. R. Reed (Johns Hopkins School of Medicine) for kindly providing the rat type IlI adenylyl cyclase cDNA. This research was supported by NIH Grants NS 20498 and HL 44948. Z.X. and EJ.C. were supported by Washington Heart Association postdoctoral fellowships.
PY - 1992/9/14
Y1 - 1992/9/14
N2 - A cDNA clone for type III adenylyl cyclase was originally isolated from a rat olfactory cDNA library and Northern analysis using total RNA suggested that the expression of the type III mRNA may be limited to the olfactory epithelium (Bakalyar and Reed, Science, 250 (1990) 1403-1406). In this study, the distribution of type III adenylyl cyclase mRNA in a number of bovine tissues and cultured cells was examined by Northern analysis using poly(A)+ RNA. Type III adenylyl cyclase mRNA was expressed in brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung, retina, 293 cells and PC-12 cells. Furthermore, the Ca2+ sensitivity of adenylyl cyclase activity in 293 cells indicated the presence of type III adenylyl cyclase. These data indicate that expression of the type III adenylyl cyclase is not limited to olfactory tissues, and that this enzyme probably has a number of physiological functions in addition to olfactory signal transduction.
AB - A cDNA clone for type III adenylyl cyclase was originally isolated from a rat olfactory cDNA library and Northern analysis using total RNA suggested that the expression of the type III mRNA may be limited to the olfactory epithelium (Bakalyar and Reed, Science, 250 (1990) 1403-1406). In this study, the distribution of type III adenylyl cyclase mRNA in a number of bovine tissues and cultured cells was examined by Northern analysis using poly(A)+ RNA. Type III adenylyl cyclase mRNA was expressed in brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung, retina, 293 cells and PC-12 cells. Furthermore, the Ca2+ sensitivity of adenylyl cyclase activity in 293 cells indicated the presence of type III adenylyl cyclase. These data indicate that expression of the type III adenylyl cyclase is not limited to olfactory tissues, and that this enzyme probably has a number of physiological functions in addition to olfactory signal transduction.
KW - Ca
KW - Calmodulin
KW - Tissue distribution
KW - Type III adenylyl cyclase
UR - http://www.scopus.com/inward/record.url?scp=0026701283&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(92)90742-P
DO - 10.1016/0304-3940(92)90742-P
M3 - Article
C2 - 1436697
AN - SCOPUS:0026701283
SN - 0304-3940
VL - 144
SP - 169
EP - 173
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1-2
ER -