The use of collagen content as determined by spectral domain polarization-sensitive optical coherence tomography to assess colon anastomosis healing in a rat model

K. H. Son, H. W. Jeong, W. W. Jung, H. S. Kim, Seung Kwan Lee, K. T. Kim, C. B. Ahn, K. Y. Park, Beop-Min Kim, Sung Ho Lee

Research output: Contribution to journalArticle

Abstract

Background/Purpose: Many studies have been undertaken to prevent anastomosis leakage of the colon, and several methods have been used to assess anastomosis healing, such as measurement of bursting pressure or hydroxyproline (a marker of collagen) content at the anastomosis site. However, these methods are inappropriate for comparing anastomosis healing at two time points in the same animals. In the present study, we measured the collagen level by spectral domain polarization-sensitive optical coherence tomography (SD-PS-OCT) to assess anastomosis healing. Methods: Sprague-Dawley rats were divided into groups C (saline-administered controls; study group) and M [a 5-fluorouracil (5-FU)-administered experimental group]. Immediately after end-to-end anastomosis of the colon, SD-PS-OCT images of anastomoses were taken (baseline). Animals were administered saline or 5-FU for 7 days. On the 7th postoperative day, SD-PS-OCT images were acquired, a histopathologic exam was performed, and hydroxyproline levels as well as mRNA expressions of collagen-1 and collagen-3 were measured at the anastomosis site. Results: Fibroblast proliferation and inflammatory cell infiltration were greater in group C than in group M. The mRNA expressions of collagen-1 and collagen-3 were substantially higher in group C. Hydroxyproline levels were higher in group M than in group C. Though collagen levels measured by SD-PS-OCT at 7 days were elevated compared with baseline in group C, no such changes were observed for group M. Conclusion: Collagen levels at the colon anastomosis site, measured with SD-PS-OCT, were not increased at 7 days postoperatively versus baseline when 5-FU was injected, but were increased in saline-treated controls. The measurement of collagen content by SD-PS-OCT was found to provide a good means of assessing anastomosis healing, because it allows in situ assessment of collagen contents at baseline and during the postoperative period.

Original languageEnglish
Pages (from-to)32-40
Number of pages9
JournalEuropean Surgical Research
Volume52
Issue number1-2
DOIs
Publication statusPublished - 2014

Fingerprint

Optical Coherence Tomography
Colon
Collagen
Hydroxyproline
Fluorouracil
Messenger RNA
Postoperative Period
Sprague Dawley Rats
Fibroblasts
Cell Proliferation
Pressure

Keywords

  • 5-Fluorouracil
  • Colon anastomosis
  • Polarization-sensitive optical coherence tomography
  • Wound healing

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)

Cite this

The use of collagen content as determined by spectral domain polarization-sensitive optical coherence tomography to assess colon anastomosis healing in a rat model. / Son, K. H.; Jeong, H. W.; Jung, W. W.; Kim, H. S.; Lee, Seung Kwan; Kim, K. T.; Ahn, C. B.; Park, K. Y.; Kim, Beop-Min; Lee, Sung Ho.

In: European Surgical Research, Vol. 52, No. 1-2, 2014, p. 32-40.

Research output: Contribution to journalArticle

@article{1a28e484a5bb455692a0fcb2996121a6,
title = "The use of collagen content as determined by spectral domain polarization-sensitive optical coherence tomography to assess colon anastomosis healing in a rat model",
abstract = "Background/Purpose: Many studies have been undertaken to prevent anastomosis leakage of the colon, and several methods have been used to assess anastomosis healing, such as measurement of bursting pressure or hydroxyproline (a marker of collagen) content at the anastomosis site. However, these methods are inappropriate for comparing anastomosis healing at two time points in the same animals. In the present study, we measured the collagen level by spectral domain polarization-sensitive optical coherence tomography (SD-PS-OCT) to assess anastomosis healing. Methods: Sprague-Dawley rats were divided into groups C (saline-administered controls; study group) and M [a 5-fluorouracil (5-FU)-administered experimental group]. Immediately after end-to-end anastomosis of the colon, SD-PS-OCT images of anastomoses were taken (baseline). Animals were administered saline or 5-FU for 7 days. On the 7th postoperative day, SD-PS-OCT images were acquired, a histopathologic exam was performed, and hydroxyproline levels as well as mRNA expressions of collagen-1 and collagen-3 were measured at the anastomosis site. Results: Fibroblast proliferation and inflammatory cell infiltration were greater in group C than in group M. The mRNA expressions of collagen-1 and collagen-3 were substantially higher in group C. Hydroxyproline levels were higher in group M than in group C. Though collagen levels measured by SD-PS-OCT at 7 days were elevated compared with baseline in group C, no such changes were observed for group M. Conclusion: Collagen levels at the colon anastomosis site, measured with SD-PS-OCT, were not increased at 7 days postoperatively versus baseline when 5-FU was injected, but were increased in saline-treated controls. The measurement of collagen content by SD-PS-OCT was found to provide a good means of assessing anastomosis healing, because it allows in situ assessment of collagen contents at baseline and during the postoperative period.",
keywords = "5-Fluorouracil, Colon anastomosis, Polarization-sensitive optical coherence tomography, Wound healing",
author = "Son, {K. H.} and Jeong, {H. W.} and Jung, {W. W.} and Kim, {H. S.} and Lee, {Seung Kwan} and Kim, {K. T.} and Ahn, {C. B.} and Park, {K. Y.} and Beop-Min Kim and Lee, {Sung Ho}",
year = "2014",
doi = "10.1159/000358057",
language = "English",
volume = "52",
pages = "32--40",
journal = "European Surgical Research",
issn = "0014-312X",
publisher = "S. Karger AG",
number = "1-2",

}

TY - JOUR

T1 - The use of collagen content as determined by spectral domain polarization-sensitive optical coherence tomography to assess colon anastomosis healing in a rat model

AU - Son, K. H.

AU - Jeong, H. W.

AU - Jung, W. W.

AU - Kim, H. S.

AU - Lee, Seung Kwan

AU - Kim, K. T.

AU - Ahn, C. B.

AU - Park, K. Y.

AU - Kim, Beop-Min

AU - Lee, Sung Ho

PY - 2014

Y1 - 2014

N2 - Background/Purpose: Many studies have been undertaken to prevent anastomosis leakage of the colon, and several methods have been used to assess anastomosis healing, such as measurement of bursting pressure or hydroxyproline (a marker of collagen) content at the anastomosis site. However, these methods are inappropriate for comparing anastomosis healing at two time points in the same animals. In the present study, we measured the collagen level by spectral domain polarization-sensitive optical coherence tomography (SD-PS-OCT) to assess anastomosis healing. Methods: Sprague-Dawley rats were divided into groups C (saline-administered controls; study group) and M [a 5-fluorouracil (5-FU)-administered experimental group]. Immediately after end-to-end anastomosis of the colon, SD-PS-OCT images of anastomoses were taken (baseline). Animals were administered saline or 5-FU for 7 days. On the 7th postoperative day, SD-PS-OCT images were acquired, a histopathologic exam was performed, and hydroxyproline levels as well as mRNA expressions of collagen-1 and collagen-3 were measured at the anastomosis site. Results: Fibroblast proliferation and inflammatory cell infiltration were greater in group C than in group M. The mRNA expressions of collagen-1 and collagen-3 were substantially higher in group C. Hydroxyproline levels were higher in group M than in group C. Though collagen levels measured by SD-PS-OCT at 7 days were elevated compared with baseline in group C, no such changes were observed for group M. Conclusion: Collagen levels at the colon anastomosis site, measured with SD-PS-OCT, were not increased at 7 days postoperatively versus baseline when 5-FU was injected, but were increased in saline-treated controls. The measurement of collagen content by SD-PS-OCT was found to provide a good means of assessing anastomosis healing, because it allows in situ assessment of collagen contents at baseline and during the postoperative period.

AB - Background/Purpose: Many studies have been undertaken to prevent anastomosis leakage of the colon, and several methods have been used to assess anastomosis healing, such as measurement of bursting pressure or hydroxyproline (a marker of collagen) content at the anastomosis site. However, these methods are inappropriate for comparing anastomosis healing at two time points in the same animals. In the present study, we measured the collagen level by spectral domain polarization-sensitive optical coherence tomography (SD-PS-OCT) to assess anastomosis healing. Methods: Sprague-Dawley rats were divided into groups C (saline-administered controls; study group) and M [a 5-fluorouracil (5-FU)-administered experimental group]. Immediately after end-to-end anastomosis of the colon, SD-PS-OCT images of anastomoses were taken (baseline). Animals were administered saline or 5-FU for 7 days. On the 7th postoperative day, SD-PS-OCT images were acquired, a histopathologic exam was performed, and hydroxyproline levels as well as mRNA expressions of collagen-1 and collagen-3 were measured at the anastomosis site. Results: Fibroblast proliferation and inflammatory cell infiltration were greater in group C than in group M. The mRNA expressions of collagen-1 and collagen-3 were substantially higher in group C. Hydroxyproline levels were higher in group M than in group C. Though collagen levels measured by SD-PS-OCT at 7 days were elevated compared with baseline in group C, no such changes were observed for group M. Conclusion: Collagen levels at the colon anastomosis site, measured with SD-PS-OCT, were not increased at 7 days postoperatively versus baseline when 5-FU was injected, but were increased in saline-treated controls. The measurement of collagen content by SD-PS-OCT was found to provide a good means of assessing anastomosis healing, because it allows in situ assessment of collagen contents at baseline and during the postoperative period.

KW - 5-Fluorouracil

KW - Colon anastomosis

KW - Polarization-sensitive optical coherence tomography

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=84893194973&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893194973&partnerID=8YFLogxK

U2 - 10.1159/000358057

DO - 10.1159/000358057

M3 - Article

VL - 52

SP - 32

EP - 40

JO - European Surgical Research

JF - European Surgical Research

SN - 0014-312X

IS - 1-2

ER -