The usefulness of cytokeratin immunohistochemistry in detection of lymph node micrometastasis in neck dissection specimens

Soon Young Kwon, Hyung Jin Kim, Jeong-Soo Woo, Kwang-Yoon Jung, Insun Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Our study was designed to find out the rate and the characteristics of micrometastasis in cervical lymph nodes using immunohistochemical staining. From 69 patients, 1710 lymph nodes negative for metastasis on hematoxylin-eosin stain, were examined. Immunohistochemical stain was performed using pan-cytokeratin AE1/AE3 antibody. In 13 cases, occult lymph node metastasis was detected by immunohistochemical method. On retrospective review of the hematoxylin-eosin stain by the pathologist, lymph node metastasis was detected in 4 of 13 patients. Because the immunohistochemical method enhanced the detection rate of occult micrometastasis in cervical lymph nodes of head and neck squamous cell carcinoma patients, it may be recommended for routine diagnostic use in patient with negative for a lymph node metastasis on routine hematoxylin-eosin stain.

Original languageEnglish
Pages (from-to)300-306
Number of pages7
JournalOtolaryngology - Head and Neck Surgery
Volume131
Issue number3
DOIs
Publication statusPublished - 2004 Sep 1

Fingerprint

Neoplasm Micrometastasis
Neck Dissection
Keratins
Lymph Nodes
Immunohistochemistry
Coloring Agents
Hematoxylin
Eosine Yellowish-(YS)
Neoplasm Metastasis
Staining and Labeling
Antibodies

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

@article{376333f1edce45c2b09b1641d689e3ef,
title = "The usefulness of cytokeratin immunohistochemistry in detection of lymph node micrometastasis in neck dissection specimens",
abstract = "Our study was designed to find out the rate and the characteristics of micrometastasis in cervical lymph nodes using immunohistochemical staining. From 69 patients, 1710 lymph nodes negative for metastasis on hematoxylin-eosin stain, were examined. Immunohistochemical stain was performed using pan-cytokeratin AE1/AE3 antibody. In 13 cases, occult lymph node metastasis was detected by immunohistochemical method. On retrospective review of the hematoxylin-eosin stain by the pathologist, lymph node metastasis was detected in 4 of 13 patients. Because the immunohistochemical method enhanced the detection rate of occult micrometastasis in cervical lymph nodes of head and neck squamous cell carcinoma patients, it may be recommended for routine diagnostic use in patient with negative for a lymph node metastasis on routine hematoxylin-eosin stain.",
author = "Kwon, {Soon Young} and Kim, {Hyung Jin} and Jeong-Soo Woo and Kwang-Yoon Jung and Insun Kim",
year = "2004",
month = "9",
day = "1",
doi = "10.1016/j.otohns.2004.02.020",
language = "English",
volume = "131",
pages = "300--306",
journal = "Otolaryngology - Head and Neck Surgery (United States)",
issn = "0194-5998",
publisher = "Mosby Inc.",
number = "3",

}

TY - JOUR

T1 - The usefulness of cytokeratin immunohistochemistry in detection of lymph node micrometastasis in neck dissection specimens

AU - Kwon, Soon Young

AU - Kim, Hyung Jin

AU - Woo, Jeong-Soo

AU - Jung, Kwang-Yoon

AU - Kim, Insun

PY - 2004/9/1

Y1 - 2004/9/1

N2 - Our study was designed to find out the rate and the characteristics of micrometastasis in cervical lymph nodes using immunohistochemical staining. From 69 patients, 1710 lymph nodes negative for metastasis on hematoxylin-eosin stain, were examined. Immunohistochemical stain was performed using pan-cytokeratin AE1/AE3 antibody. In 13 cases, occult lymph node metastasis was detected by immunohistochemical method. On retrospective review of the hematoxylin-eosin stain by the pathologist, lymph node metastasis was detected in 4 of 13 patients. Because the immunohistochemical method enhanced the detection rate of occult micrometastasis in cervical lymph nodes of head and neck squamous cell carcinoma patients, it may be recommended for routine diagnostic use in patient with negative for a lymph node metastasis on routine hematoxylin-eosin stain.

AB - Our study was designed to find out the rate and the characteristics of micrometastasis in cervical lymph nodes using immunohistochemical staining. From 69 patients, 1710 lymph nodes negative for metastasis on hematoxylin-eosin stain, were examined. Immunohistochemical stain was performed using pan-cytokeratin AE1/AE3 antibody. In 13 cases, occult lymph node metastasis was detected by immunohistochemical method. On retrospective review of the hematoxylin-eosin stain by the pathologist, lymph node metastasis was detected in 4 of 13 patients. Because the immunohistochemical method enhanced the detection rate of occult micrometastasis in cervical lymph nodes of head and neck squamous cell carcinoma patients, it may be recommended for routine diagnostic use in patient with negative for a lymph node metastasis on routine hematoxylin-eosin stain.

UR - http://www.scopus.com/inward/record.url?scp=4444254850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444254850&partnerID=8YFLogxK

U2 - 10.1016/j.otohns.2004.02.020

DO - 10.1016/j.otohns.2004.02.020

M3 - Article

C2 - 15365551

AN - SCOPUS:4444254850

VL - 131

SP - 300

EP - 306

JO - Otolaryngology - Head and Neck Surgery (United States)

JF - Otolaryngology - Head and Neck Surgery (United States)

SN - 0194-5998

IS - 3

ER -