Abstract
Background: Immunohistochemical staining for p16INK4a and Ki-67 has been used to improve the accuracy in making a diagnosis of the uterine cervix cancer on biopsy. This study was conducted to examine the usefulness of these markers in the pathological diagnosis based on cervical biopsy. Methods: We selected a consecutive series of 111 colposcopically directed cervical punch biopsies. Using these biopsy samples, we performed an immunohistochemical staining for p16INK4a and Ki-67 to establish a diagnosis. The slides were circulated among four pathologists in a sequential order: the hematoxylin and eosin (H&E) slide, H&E slide and p16INK4a-stained slide, and H&E slide, p16INK4a- and Ki-67-stained slides. Results: The overall rates of the concordance in the first, the second, and the third diagnoses were 77.5%, 82.0%, and 82.0%, respectively. The rate of the concordance in the diagnosis of cervical intraepithelial neoplasm (CIN) 2/3 was increased from 62.2% to 73.0%. But there was a variability in the rate of the revision of the diagnosis between the pathologists. With the application of criteria for interpreting the expressions of p16INK4a and Ki-67, benign and CIN 1 lesions showed a p16INK4a expression score of 0 or 1. But CIN 2 and CIN 3 lesions showed a p16INK4a expression score of 2 and 3, respectively. Conclusions: The immunostain for p16INK4a and Ki-67 might be useful in reducing an inter-observer variability. But criteria for interpreting both markers should be strictly applied.
| Original language | English |
|---|---|
| Pages (from-to) | 253-260 |
| Number of pages | 8 |
| Journal | Korean Journal of Pathology |
| Volume | 46 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2012 Oct 30 |
| Externally published | Yes |
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Keywords
- Ki-67
- P16INK4a
- Uterine cervix
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
The utility of p16INK4a and Ki-67 as a conjunctive tool in uterine cervical lesions. / Lee, Sangho; Kim, Hyunchul; Kim, Hyesun; Kim, Chul Hwan; Kim, Insun.
In: Korean Journal of Pathology, Vol. 46, No. 3, 30.10.2012, p. 253-260.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The utility of p16INK4a and Ki-67 as a conjunctive tool in uterine cervical lesions
AU - Lee, Sangho
AU - Kim, Hyunchul
AU - Kim, Hyesun
AU - Kim, Chul Hwan
AU - Kim, Insun
PY - 2012/10/30
Y1 - 2012/10/30
N2 - Background: Immunohistochemical staining for p16INK4a and Ki-67 has been used to improve the accuracy in making a diagnosis of the uterine cervix cancer on biopsy. This study was conducted to examine the usefulness of these markers in the pathological diagnosis based on cervical biopsy. Methods: We selected a consecutive series of 111 colposcopically directed cervical punch biopsies. Using these biopsy samples, we performed an immunohistochemical staining for p16INK4a and Ki-67 to establish a diagnosis. The slides were circulated among four pathologists in a sequential order: the hematoxylin and eosin (H&E) slide, H&E slide and p16INK4a-stained slide, and H&E slide, p16INK4a- and Ki-67-stained slides. Results: The overall rates of the concordance in the first, the second, and the third diagnoses were 77.5%, 82.0%, and 82.0%, respectively. The rate of the concordance in the diagnosis of cervical intraepithelial neoplasm (CIN) 2/3 was increased from 62.2% to 73.0%. But there was a variability in the rate of the revision of the diagnosis between the pathologists. With the application of criteria for interpreting the expressions of p16INK4a and Ki-67, benign and CIN 1 lesions showed a p16INK4a expression score of 0 or 1. But CIN 2 and CIN 3 lesions showed a p16INK4a expression score of 2 and 3, respectively. Conclusions: The immunostain for p16INK4a and Ki-67 might be useful in reducing an inter-observer variability. But criteria for interpreting both markers should be strictly applied.
AB - Background: Immunohistochemical staining for p16INK4a and Ki-67 has been used to improve the accuracy in making a diagnosis of the uterine cervix cancer on biopsy. This study was conducted to examine the usefulness of these markers in the pathological diagnosis based on cervical biopsy. Methods: We selected a consecutive series of 111 colposcopically directed cervical punch biopsies. Using these biopsy samples, we performed an immunohistochemical staining for p16INK4a and Ki-67 to establish a diagnosis. The slides were circulated among four pathologists in a sequential order: the hematoxylin and eosin (H&E) slide, H&E slide and p16INK4a-stained slide, and H&E slide, p16INK4a- and Ki-67-stained slides. Results: The overall rates of the concordance in the first, the second, and the third diagnoses were 77.5%, 82.0%, and 82.0%, respectively. The rate of the concordance in the diagnosis of cervical intraepithelial neoplasm (CIN) 2/3 was increased from 62.2% to 73.0%. But there was a variability in the rate of the revision of the diagnosis between the pathologists. With the application of criteria for interpreting the expressions of p16INK4a and Ki-67, benign and CIN 1 lesions showed a p16INK4a expression score of 0 or 1. But CIN 2 and CIN 3 lesions showed a p16INK4a expression score of 2 and 3, respectively. Conclusions: The immunostain for p16INK4a and Ki-67 might be useful in reducing an inter-observer variability. But criteria for interpreting both markers should be strictly applied.
KW - Ki-67
KW - P16INK4a
KW - Uterine cervix
UR - http://www.scopus.com/inward/record.url?scp=84867849896&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867849896&partnerID=8YFLogxK
U2 - 10.4132/KoreanJPathol.2012.46.3.253
DO - 10.4132/KoreanJPathol.2012.46.3.253
M3 - Article
C2 - 23110011
AN - SCOPUS:84867849896
VL - 46
SP - 253
EP - 260
JO - Journal of Pathology and Translational Medicine
JF - Journal of Pathology and Translational Medicine
SN - 2383-7837
IS - 3
ER -