Therapeutic effects and immunomodulation of suanbo mineral water therapy in a murine model of atopic dermatitis

Yoon Jung Choi, Hye Jin Lee, Do Hyun Lee, So Youn Woo, Kyung Ho Lee, Seong Taek Yun, Jong Moon Kim, Hong Jig Kim, Jin Wou Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Balneotherapy is widely used as an alternative treatment modality for AD. Although the clinical benefit of some mineral waters has been established, their mechanisms of action in alleviating AD are only partly understood. Objective: The clinical modification and immunomodulatory or anti- inflammatory effects of mineral water from the Suanbo hot springs on the differentiation and cytokine production of Th1, Th2, and regulatory T cells (Treg) were investigated using spleen, skin tissue, and serum from NC/Nga mice. Methods: The therapeutic effects of bathing in mineral water in a Dermatophagoides farinae body extract ointment (Dfb ointment)-induced AD mouse model were assessed by measuring the modified Scoring atopic dermatitis (SCORAD) index scores, transepidermal water loss (TEWL), histological and immunohistochemical changes of the skin lesion, serum levels of interferon (IFN)-γ, interleukin (IL)-4, IL-5 and immunoglobulin E, mRNA expression of IFN-γ, IL-4 and IL-5 of dorsal skin, and helper T cell differentiation in the spleen. Results: Bathing in mineral water significantly reduced the modified SCORAD index scores, TEWL, epidermal hyperplasia, and inflammatory cell infiltration. IL-4 production and Th2 cell differentiation showed a decreasing tendency with mineral water bathing, but the Th1 cells did not. On the contrary, differentiation to Treg cells was promoted with mineral water bathing. Conclusion: Balneotherapy not only has anti-inflammatory activity, but also shows positive effects on cutaneous barrier homeostasis. These results suggest that the favorable effects of balneotherapy may be mediated by modifying the Th2 response, and possibly in part by inducing Treg cell differentiation. (Ann Dermatol 25(4) 462-470, 2013).

Original languageEnglish
Pages (from-to)462-470
Number of pages9
JournalAnnals of Dermatology
Volume25
Issue number4
DOIs
Publication statusPublished - 2013 Nov 1

Fingerprint

Mineral Waters
Immunomodulation
Therapeutic Uses
Atopic Dermatitis
Balneology
Regulatory T-Lymphocytes
Interleukin-4
Cell Differentiation
Skin
Interleukin-5
Interferons
Anti-Inflammatory Agents
Therapeutics
Spleen
Dermatophagoides farinae
Hot Springs
Th2 Cells
Th1 Cells
Water
Ointments

Keywords

  • Atopic dermatitis
  • Balneotherapy
  • Mineral waters
  • NC/NGa mice

ASJC Scopus subject areas

  • Dermatology

Cite this

Therapeutic effects and immunomodulation of suanbo mineral water therapy in a murine model of atopic dermatitis. / Choi, Yoon Jung; Lee, Hye Jin; Lee, Do Hyun; Woo, So Youn; Lee, Kyung Ho; Yun, Seong Taek; Kim, Jong Moon; Kim, Hong Jig; Kim, Jin Wou.

In: Annals of Dermatology, Vol. 25, No. 4, 01.11.2013, p. 462-470.

Research output: Contribution to journalArticle

Choi, Yoon Jung ; Lee, Hye Jin ; Lee, Do Hyun ; Woo, So Youn ; Lee, Kyung Ho ; Yun, Seong Taek ; Kim, Jong Moon ; Kim, Hong Jig ; Kim, Jin Wou. / Therapeutic effects and immunomodulation of suanbo mineral water therapy in a murine model of atopic dermatitis. In: Annals of Dermatology. 2013 ; Vol. 25, No. 4. pp. 462-470.
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abstract = "Background: Balneotherapy is widely used as an alternative treatment modality for AD. Although the clinical benefit of some mineral waters has been established, their mechanisms of action in alleviating AD are only partly understood. Objective: The clinical modification and immunomodulatory or anti- inflammatory effects of mineral water from the Suanbo hot springs on the differentiation and cytokine production of Th1, Th2, and regulatory T cells (Treg) were investigated using spleen, skin tissue, and serum from NC/Nga mice. Methods: The therapeutic effects of bathing in mineral water in a Dermatophagoides farinae body extract ointment (Dfb ointment)-induced AD mouse model were assessed by measuring the modified Scoring atopic dermatitis (SCORAD) index scores, transepidermal water loss (TEWL), histological and immunohistochemical changes of the skin lesion, serum levels of interferon (IFN)-γ, interleukin (IL)-4, IL-5 and immunoglobulin E, mRNA expression of IFN-γ, IL-4 and IL-5 of dorsal skin, and helper T cell differentiation in the spleen. Results: Bathing in mineral water significantly reduced the modified SCORAD index scores, TEWL, epidermal hyperplasia, and inflammatory cell infiltration. IL-4 production and Th2 cell differentiation showed a decreasing tendency with mineral water bathing, but the Th1 cells did not. On the contrary, differentiation to Treg cells was promoted with mineral water bathing. Conclusion: Balneotherapy not only has anti-inflammatory activity, but also shows positive effects on cutaneous barrier homeostasis. These results suggest that the favorable effects of balneotherapy may be mediated by modifying the Th2 response, and possibly in part by inducing Treg cell differentiation. (Ann Dermatol 25(4) 462-470, 2013).",
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AU - Choi, Yoon Jung

AU - Lee, Hye Jin

AU - Lee, Do Hyun

AU - Woo, So Youn

AU - Lee, Kyung Ho

AU - Yun, Seong Taek

AU - Kim, Jong Moon

AU - Kim, Hong Jig

AU - Kim, Jin Wou

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N2 - Background: Balneotherapy is widely used as an alternative treatment modality for AD. Although the clinical benefit of some mineral waters has been established, their mechanisms of action in alleviating AD are only partly understood. Objective: The clinical modification and immunomodulatory or anti- inflammatory effects of mineral water from the Suanbo hot springs on the differentiation and cytokine production of Th1, Th2, and regulatory T cells (Treg) were investigated using spleen, skin tissue, and serum from NC/Nga mice. Methods: The therapeutic effects of bathing in mineral water in a Dermatophagoides farinae body extract ointment (Dfb ointment)-induced AD mouse model were assessed by measuring the modified Scoring atopic dermatitis (SCORAD) index scores, transepidermal water loss (TEWL), histological and immunohistochemical changes of the skin lesion, serum levels of interferon (IFN)-γ, interleukin (IL)-4, IL-5 and immunoglobulin E, mRNA expression of IFN-γ, IL-4 and IL-5 of dorsal skin, and helper T cell differentiation in the spleen. Results: Bathing in mineral water significantly reduced the modified SCORAD index scores, TEWL, epidermal hyperplasia, and inflammatory cell infiltration. IL-4 production and Th2 cell differentiation showed a decreasing tendency with mineral water bathing, but the Th1 cells did not. On the contrary, differentiation to Treg cells was promoted with mineral water bathing. Conclusion: Balneotherapy not only has anti-inflammatory activity, but also shows positive effects on cutaneous barrier homeostasis. These results suggest that the favorable effects of balneotherapy may be mediated by modifying the Th2 response, and possibly in part by inducing Treg cell differentiation. (Ann Dermatol 25(4) 462-470, 2013).

AB - Background: Balneotherapy is widely used as an alternative treatment modality for AD. Although the clinical benefit of some mineral waters has been established, their mechanisms of action in alleviating AD are only partly understood. Objective: The clinical modification and immunomodulatory or anti- inflammatory effects of mineral water from the Suanbo hot springs on the differentiation and cytokine production of Th1, Th2, and regulatory T cells (Treg) were investigated using spleen, skin tissue, and serum from NC/Nga mice. Methods: The therapeutic effects of bathing in mineral water in a Dermatophagoides farinae body extract ointment (Dfb ointment)-induced AD mouse model were assessed by measuring the modified Scoring atopic dermatitis (SCORAD) index scores, transepidermal water loss (TEWL), histological and immunohistochemical changes of the skin lesion, serum levels of interferon (IFN)-γ, interleukin (IL)-4, IL-5 and immunoglobulin E, mRNA expression of IFN-γ, IL-4 and IL-5 of dorsal skin, and helper T cell differentiation in the spleen. Results: Bathing in mineral water significantly reduced the modified SCORAD index scores, TEWL, epidermal hyperplasia, and inflammatory cell infiltration. IL-4 production and Th2 cell differentiation showed a decreasing tendency with mineral water bathing, but the Th1 cells did not. On the contrary, differentiation to Treg cells was promoted with mineral water bathing. Conclusion: Balneotherapy not only has anti-inflammatory activity, but also shows positive effects on cutaneous barrier homeostasis. These results suggest that the favorable effects of balneotherapy may be mediated by modifying the Th2 response, and possibly in part by inducing Treg cell differentiation. (Ann Dermatol 25(4) 462-470, 2013).

KW - Atopic dermatitis

KW - Balneotherapy

KW - Mineral waters

KW - NC/NGa mice

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