Thermal Denaturation of Tilapia Myosin and Its Subunits as Affected by Constantly Increasing Temperature

Zachary Harold Reed, William Guilford, Jae W. Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purified tilapia myosin was digested with α-chymotrypsin and purified to obtain heavy meromyosin (HMM) and light meromyosin (LMM). Biochemical properties of tilapia myosin, HMM, and LMM were characterized. Surface hydrophobicity (S o) showed an increase for myosin and HMM between 30 and 40 °C and reached a plateau at 70 °C. LMM, in a small magnitude, also showed a continuous increase to 70 °C. Total sulfhydryl content (TSH) demonstrated that the SH residue content of HMM was nearly double that of LMM. Surface reactive sulfhydryl groups (SRSH) for myosin and HMM were relatively unchanged from 10 to 30 °C but increased from 30 to 50 °C. The exposure of buried hydrophobic and sulfhydryl groups of myosin and HMM increased as the myosin and HMM were constantly heated. However, the TSH and SRSH results indicated that the stability of LMM was likely due to its α-helix conformation. Reducing and nonreducing sodium dodecylsulfate-polyacryamide gel electrophoresis helped to understand the role of disulfide bonds in thermal aggregation of tilapia myosin, HMM, and LMM.

Original languageEnglish
JournalJournal of Food Science
Volume76
Issue number7
DOIs
Publication statusPublished - 2011 Sep 1
Externally publishedYes

Fingerprint

heavy meromyosin
Myosin Subfragments
Tilapia
Myosins
myosin
denaturation
Hot Temperature
heat
Temperature
sulfhydryl groups
temperature
photostability
disulfide bonds
chymotrypsin
hydrophobicity
gel electrophoresis
plateaus
sodium

ASJC Scopus subject areas

  • Food Science

Cite this

Thermal Denaturation of Tilapia Myosin and Its Subunits as Affected by Constantly Increasing Temperature. / Reed, Zachary Harold; Guilford, William; Park, Jae W.

In: Journal of Food Science, Vol. 76, No. 7, 01.09.2011.

Research output: Contribution to journalArticle

Reed, Zachary Harold ; Guilford, William ; Park, Jae W. / Thermal Denaturation of Tilapia Myosin and Its Subunits as Affected by Constantly Increasing Temperature. In: Journal of Food Science. 2011 ; Vol. 76, No. 7.
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