Thermodynamically controlled separation of polyvalent 2-nm gold nanoparticle-oligonucleotide conjugates

Jae-Seung Lee, Dwight S. Seferos, David A. Giljohann, Chad A. Mirkin

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We describe the synthesis of small (2-nm diameter) gold nanoparticles densely functionalized with thiolated DNA (DNA-Au NPs) and a method to separate these particles from excess free DNA after synthesis. The separation method utilizes the thermodynamically enhanced binding properties of 2-nm DNA-Au NPs, compared to free excess DNA, to selectively hybridize these small particles to larger (15-nm diameter) DNA-Au NPs and form aggregates that can be isolated by simple centrifugation. These 2-nm DNA-Au NPs are obtained in a 46% overall yield, have a high surface coverage of DNA (64.8 ± 6.4 pmol/cm2), and as a result, exhibit increased melting temperatures and cooperative melting properties.

Original languageEnglish
Pages (from-to)5430-5431
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number16
DOIs
Publication statusPublished - 2008 Apr 23
Externally publishedYes

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Oligonucleotides
Gold
Nanoparticles
DNA
Freezing
Centrifugation
Melting point
Melting
Temperature

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Thermodynamically controlled separation of polyvalent 2-nm gold nanoparticle-oligonucleotide conjugates. / Lee, Jae-Seung; Seferos, Dwight S.; Giljohann, David A.; Mirkin, Chad A.

In: Journal of the American Chemical Society, Vol. 130, No. 16, 23.04.2008, p. 5430-5431.

Research output: Contribution to journalArticle

Lee, Jae-Seung ; Seferos, Dwight S. ; Giljohann, David A. ; Mirkin, Chad A. / Thermodynamically controlled separation of polyvalent 2-nm gold nanoparticle-oligonucleotide conjugates. In: Journal of the American Chemical Society. 2008 ; Vol. 130, No. 16. pp. 5430-5431.
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