Thermolabile alanine racemase from a psychotroph, Pseudomonas fluorescens

purification and properties.

K. Yokoigawa, H. Kawai, K. Endo, Young Hee Lim, N. Esaki, K. Soda

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A psychotrophic bacterium that produces a thermolabile alanine racemase was isolated from raw milk, and identified as Pseudomonas fluorescens TM5-2. The enzyme was purified to homogeneity from the cell extract, and characterized to be compared with enzymes from mesophiles (Bacillus subtilis and Salmonella typhimurium) and a thermophile (Bacillus stearothermophilus). The enzyme has a molecular weight of about 76,000 and consists of two subunits identical in molecular weight (38,000). The enzyme contains two mol of pyridoxal 5'-phosphate per mol as a coenzyme. The amino acid composition was different from those of other alanine racemases in content of valine. The amino acid sequence of the amino terminal region (from 1Met to 25Gly) had 21-33% homology with those of other alanine racemases. Kinetic parameters of the enzyme were similar to those of other alanine racemases. The enzyme is extremely labile over 30 degrees C, and shows the high catalytic activity even at 0 degrees C; it is thermolabile and psychotrophic.

Original languageEnglish
Pages (from-to)93-97
Number of pages5
JournalBioscience, biotechnology, and biochemistry
Volume57
Issue number1
Publication statusPublished - 1993 Jan 1
Externally publishedYes

Fingerprint

Alanine Racemase
Pseudomonas fluorescens
alanine
Purification
Enzymes
enzymes
Bacilli
thermophilic microorganisms
Molecular Weight
Molecular weight
molecular weight
pyridoxal phosphate
Geobacillus stearothermophilus
Amino Acids
Salmonella
Pyridoxal Phosphate
coenzymes
Coenzymes
Valine
Salmonella typhimurium

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology

Cite this

Thermolabile alanine racemase from a psychotroph, Pseudomonas fluorescens : purification and properties. / Yokoigawa, K.; Kawai, H.; Endo, K.; Lim, Young Hee; Esaki, N.; Soda, K.

In: Bioscience, biotechnology, and biochemistry, Vol. 57, No. 1, 01.01.1993, p. 93-97.

Research output: Contribution to journalArticle

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