Thiamine attenuates hypoxia-induced cell death in cultured neonatal rat cardiomyocytes

Bo Hee Shin, Seung Hyuk Choi, Eun Young Cho, Min-Jeong Shin, Ki Chul Hwang, Hong Keun Cho, Ji Hyung Chung, Yangsoo Jang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Previous studies have demonstrated that thiamine (vitamin B1) has a cytoprotective effect against ischemic damage to the heart, and that heat shock protein 70 (Hsp70) is capable of protecting cardiac cells from lethal ischemia/hypoxia. We show here that thiamine has a cytoprotective effect on cultured neonatal rat cardiomyocytes under hypoxic insult, and also protects the cardiomyocytes against hypoxia-induced apoptosis; caspase-3 activation, PARP cleavage and DNA fragmentation are all inhibited. Moreover, it increases the level of Hsp70 protein in the cardiomyocytes even under prolonged hypoxic stress and its effects on hypoxia-induced cardiac cell death are antagonized by an Hsp70 inhibitor. These results suggest that the cytoprotective effect of thiamine in cardiomyocytes under hypoxic stress is due to its ability to induce Hsp70.

Original languageEnglish
Pages (from-to)133-140
Number of pages8
JournalMolecules and Cells
Volume18
Issue number2
Publication statusPublished - 2004 Oct 31
Externally publishedYes

Fingerprint

HSP70 Heat-Shock Proteins
Thiamine
Cardiac Myocytes
Cell Death
DNA Fragmentation
Caspase 3
Ischemia
Apoptosis
Hypoxia
Proteins

Keywords

  • Aging
  • Cardiomyocytes
  • Heat Shock Protein
  • Hypoxia
  • Thiamine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Shin, B. H., Choi, S. H., Cho, E. Y., Shin, M-J., Hwang, K. C., Cho, H. K., ... Jang, Y. (2004). Thiamine attenuates hypoxia-induced cell death in cultured neonatal rat cardiomyocytes. Molecules and Cells, 18(2), 133-140.

Thiamine attenuates hypoxia-induced cell death in cultured neonatal rat cardiomyocytes. / Shin, Bo Hee; Choi, Seung Hyuk; Cho, Eun Young; Shin, Min-Jeong; Hwang, Ki Chul; Cho, Hong Keun; Chung, Ji Hyung; Jang, Yangsoo.

In: Molecules and Cells, Vol. 18, No. 2, 31.10.2004, p. 133-140.

Research output: Contribution to journalArticle

Shin, BH, Choi, SH, Cho, EY, Shin, M-J, Hwang, KC, Cho, HK, Chung, JH & Jang, Y 2004, 'Thiamine attenuates hypoxia-induced cell death in cultured neonatal rat cardiomyocytes', Molecules and Cells, vol. 18, no. 2, pp. 133-140.
Shin BH, Choi SH, Cho EY, Shin M-J, Hwang KC, Cho HK et al. Thiamine attenuates hypoxia-induced cell death in cultured neonatal rat cardiomyocytes. Molecules and Cells. 2004 Oct 31;18(2):133-140.
Shin, Bo Hee ; Choi, Seung Hyuk ; Cho, Eun Young ; Shin, Min-Jeong ; Hwang, Ki Chul ; Cho, Hong Keun ; Chung, Ji Hyung ; Jang, Yangsoo. / Thiamine attenuates hypoxia-induced cell death in cultured neonatal rat cardiomyocytes. In: Molecules and Cells. 2004 ; Vol. 18, No. 2. pp. 133-140.
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