Thimerosal stimulates focal adhesion kinase and cytoskeletal changes by redox modulation

Euikyung Kim, Jung Hwan Kim, Hyeon Soo Kim, Sung Ho Ryu, Pann Ghill Suh

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14 Citations (Scopus)

Abstract

Thimerosal is one of the most widely used preservatives and has been reported to cause chemically mediated side effects. However, the mechanism of the side effects is not clearly understood yet. In the present study, we showed that HeLa S cells treated by thimerosal generated reactive oxygen species (ROS). Thimerosal-generated ROS stimulated the tyrosine phosphorylation of focal adhesion kinase (FAK) and also induced cytoskeletal changes. Pretreatment with intracellular calcium chelator, BAPTA did not block the thimerosal-mediated FAK tyrosine phosphorylation. On the other hand, either FAK inhibitor, tyrphostin or ROS scavenger, N-acetyl-L-cysteine (NAC) suppressed the tyrosine phosphorylation and cytoskeletal changes. These results suggest that thimerosal seems to induce FAK tyrosine phosphorylation and cytoskeletal changes by ROS generation but not by intracellular calcium mobilization. We think the present finding can be an important clue to understanding the mechanism of thimerosal-mediated side effects, such as contact dermatitis, and allergy.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1593
Issue number1
DOIs
Publication statusPublished - 2002 Dec 16
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

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