Three classes of mutations in the A subunit of the CCAAT-binding factor CBF delineate functional domains involved in the three-step assembly of the CBF-DNA complex

Satrajit Sinha, In-San Kim, Kun Young Sohn, Benoit De Crombrugghe, Sankar N. Maity

Research output: Contribution to journalArticle

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Abstract

The mammalian CCAAT-binding factor CBF (also called NF-Y or CP1) consists of three subunits, CBF-A, CBF-B, and CBF-C, all of which are required for DNA binding and present in the CBF-DNA complex. In this study we first established the stoichiometrics of the CBF subunits, both in the CBF molecule and in the CBF-DNA complex, and showed that one molecule of each subunit is present in the complex. To begin to understand the interactions between the CBF subunits and DNA, we performed a mutational analysis of the CBF-A subunit. This analysis identified three classes of mutations in the segment of CBF-A that is conserved in Saccharomyces cerevisiae and mammals. Analysis of the first class of mutants revealed that a major part of the conserved segment was essential for interactions with CBF-C to form a heterodimeric CBF-A/CBF-C complex. The second class of mutants identified a segment of CBF-A that is necessary for interactions between the CBF-A/CBF-C heterodimer and CBF-B to form a CBF heterotrimer. The third class defined a domain of CBF-A involved in binding the CBF heterotrimer to DNA. The second and third classes of mutants acted as dominant negative mutants inhibiting the formation of a complex between the wild-type CBF subunits and DNA. The segment of CBF-A necessary for DNA binding showed sequence homology to a segment of CBF-C. Interestingly, these sequences in CBF-A and CBF-C were also homologous to the sequences in the histone-fold motifs of histones H2B and H2A, respectively, and to the archaebacterial histone-like protein HMf-2. We discuss the functional domains of CBF-A and the properties of CBF in light of these sequence homologies and propose that an ancient histone-like motif in two CBF subunits controls the formation of a heterodimer between these subunits and the assembly of a sequence-specific DNA-protein complex.

Original languageEnglish
Pages (from-to)328-337
Number of pages10
JournalMolecular and Cellular Biology
Volume16
Issue number1
DOIs
Publication statusPublished - 1996 Jan 1
Externally publishedYes

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CCAAT-Binding Factor
Mutation
DNA
Histones
Sequence Homology
Saccharomyces cerevisiae
Mammals
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Three classes of mutations in the A subunit of the CCAAT-binding factor CBF delineate functional domains involved in the three-step assembly of the CBF-DNA complex. / Sinha, Satrajit; Kim, In-San; Sohn, Kun Young; De Crombrugghe, Benoit; Maity, Sankar N.

In: Molecular and Cellular Biology, Vol. 16, No. 1, 01.01.1996, p. 328-337.

Research output: Contribution to journalArticle

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