Abstract
We present a direct cell printing technique to pattern neural cells in a three-dimensional (3D) multilayered collagen gel. A layer of collagen precursor was printed to provide a scaffold for the cells, and the rat embryonic neurons and astrocytes were subsequently printed on the layer. A solution of sodium bicarbonate was applied to the cell containing collagen layer as nebulized aerosols, which allowed the gelation of the collagen. This process was repeated layer-by-layer to construct the 3D cell-hydrogel composites. Upon characterizing the relationship between printing resolutions and the growth of printed neural cells, single/multiple layers of neural cell-hydrogel composites were constructed and cultured. The on-demand capability to print neural cells in a multilayered hydrogel scaffold offers flexibility in generating artificial 3D neural tissue composites.
Original language | English |
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Pages (from-to) | 798-803 |
Number of pages | 6 |
Journal | Neuroreport |
Volume | 20 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2009 May 27 |
Externally published | Yes |
Keywords
- Astrocytes
- Cell printing
- Collagen hydrogel
- Freeform fabrication
- Neurons
- Tissue engineering
ASJC Scopus subject areas
- Neuroscience(all)