Three-dimensional bioprinting of rat embryonic neural cells

Wonhye Lee, Jason Pinckney, Vivian Lee, Jong-Hwan Lee, Krisztina Fischer, Samuel Polio, Je Kyun Park, Seung Schik Yoo

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

We present a direct cell printing technique to pattern neural cells in a three-dimensional (3D) multilayered collagen gel. A layer of collagen precursor was printed to provide a scaffold for the cells, and the rat embryonic neurons and astrocytes were subsequently printed on the layer. A solution of sodium bicarbonate was applied to the cell containing collagen layer as nebulized aerosols, which allowed the gelation of the collagen. This process was repeated layer-by-layer to construct the 3D cell-hydrogel composites. Upon characterizing the relationship between printing resolutions and the growth of printed neural cells, single/multiple layers of neural cell-hydrogel composites were constructed and cultured. The on-demand capability to print neural cells in a multilayered hydrogel scaffold offers flexibility in generating artificial 3D neural tissue composites.

Original languageEnglish
Pages (from-to)798-803
Number of pages6
JournalNeuroReport
Volume20
Issue number8
DOIs
Publication statusPublished - 2009 May 27
Externally publishedYes

Fingerprint

Bioprinting
Hydrogel
Collagen
Printing
Sodium Bicarbonate
Aerosols
Astrocytes
Gels

Keywords

  • Astrocytes
  • Cell printing
  • Collagen hydrogel
  • Freeform fabrication
  • Neurons
  • Tissue engineering

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Lee, W., Pinckney, J., Lee, V., Lee, J-H., Fischer, K., Polio, S., ... Yoo, S. S. (2009). Three-dimensional bioprinting of rat embryonic neural cells. NeuroReport, 20(8), 798-803. https://doi.org/10.1097/WNR.0b013e32832b8be4

Three-dimensional bioprinting of rat embryonic neural cells. / Lee, Wonhye; Pinckney, Jason; Lee, Vivian; Lee, Jong-Hwan; Fischer, Krisztina; Polio, Samuel; Park, Je Kyun; Yoo, Seung Schik.

In: NeuroReport, Vol. 20, No. 8, 27.05.2009, p. 798-803.

Research output: Contribution to journalArticle

Lee, W, Pinckney, J, Lee, V, Lee, J-H, Fischer, K, Polio, S, Park, JK & Yoo, SS 2009, 'Three-dimensional bioprinting of rat embryonic neural cells', NeuroReport, vol. 20, no. 8, pp. 798-803. https://doi.org/10.1097/WNR.0b013e32832b8be4
Lee W, Pinckney J, Lee V, Lee J-H, Fischer K, Polio S et al. Three-dimensional bioprinting of rat embryonic neural cells. NeuroReport. 2009 May 27;20(8):798-803. https://doi.org/10.1097/WNR.0b013e32832b8be4
Lee, Wonhye ; Pinckney, Jason ; Lee, Vivian ; Lee, Jong-Hwan ; Fischer, Krisztina ; Polio, Samuel ; Park, Je Kyun ; Yoo, Seung Schik. / Three-dimensional bioprinting of rat embryonic neural cells. In: NeuroReport. 2009 ; Vol. 20, No. 8. pp. 798-803.
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