Abstract
We present a direct cell printing technique to pattern neural cells in a three-dimensional (3D) multilayered collagen gel. A layer of collagen precursor was printed to provide a scaffold for the cells, and the rat embryonic neurons and astrocytes were subsequently printed on the layer. A solution of sodium bicarbonate was applied to the cell containing collagen layer as nebulized aerosols, which allowed the gelation of the collagen. This process was repeated layer-by-layer to construct the 3D cell-hydrogel composites. Upon characterizing the relationship between printing resolutions and the growth of printed neural cells, single/multiple layers of neural cell-hydrogel composites were constructed and cultured. The on-demand capability to print neural cells in a multilayered hydrogel scaffold offers flexibility in generating artificial 3D neural tissue composites.
| Original language | English |
|---|---|
| Pages (from-to) | 798-803 |
| Number of pages | 6 |
| Journal | NeuroReport |
| Volume | 20 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2009 May 27 |
| Externally published | Yes |
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Keywords
- Astrocytes
- Cell printing
- Collagen hydrogel
- Freeform fabrication
- Neurons
- Tissue engineering
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Three-dimensional bioprinting of rat embryonic neural cells. / Lee, Wonhye; Pinckney, Jason; Lee, Vivian; Lee, Jong-Hwan; Fischer, Krisztina; Polio, Samuel; Park, Je Kyun; Yoo, Seung Schik.
In: NeuroReport, Vol. 20, No. 8, 27.05.2009, p. 798-803.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Three-dimensional bioprinting of rat embryonic neural cells
AU - Lee, Wonhye
AU - Pinckney, Jason
AU - Lee, Vivian
AU - Lee, Jong-Hwan
AU - Fischer, Krisztina
AU - Polio, Samuel
AU - Park, Je Kyun
AU - Yoo, Seung Schik
PY - 2009/5/27
Y1 - 2009/5/27
N2 - We present a direct cell printing technique to pattern neural cells in a three-dimensional (3D) multilayered collagen gel. A layer of collagen precursor was printed to provide a scaffold for the cells, and the rat embryonic neurons and astrocytes were subsequently printed on the layer. A solution of sodium bicarbonate was applied to the cell containing collagen layer as nebulized aerosols, which allowed the gelation of the collagen. This process was repeated layer-by-layer to construct the 3D cell-hydrogel composites. Upon characterizing the relationship between printing resolutions and the growth of printed neural cells, single/multiple layers of neural cell-hydrogel composites were constructed and cultured. The on-demand capability to print neural cells in a multilayered hydrogel scaffold offers flexibility in generating artificial 3D neural tissue composites.
AB - We present a direct cell printing technique to pattern neural cells in a three-dimensional (3D) multilayered collagen gel. A layer of collagen precursor was printed to provide a scaffold for the cells, and the rat embryonic neurons and astrocytes were subsequently printed on the layer. A solution of sodium bicarbonate was applied to the cell containing collagen layer as nebulized aerosols, which allowed the gelation of the collagen. This process was repeated layer-by-layer to construct the 3D cell-hydrogel composites. Upon characterizing the relationship between printing resolutions and the growth of printed neural cells, single/multiple layers of neural cell-hydrogel composites were constructed and cultured. The on-demand capability to print neural cells in a multilayered hydrogel scaffold offers flexibility in generating artificial 3D neural tissue composites.
KW - Astrocytes
KW - Cell printing
KW - Collagen hydrogel
KW - Freeform fabrication
KW - Neurons
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=67649669884&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67649669884&partnerID=8YFLogxK
U2 - 10.1097/WNR.0b013e32832b8be4
DO - 10.1097/WNR.0b013e32832b8be4
M3 - Article
C2 - 19369905
AN - SCOPUS:67649669884
VL - 20
SP - 798
EP - 803
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 8
ER -