TY - GEN
T1 - Three-dimensional cell-hydrogel printer using electromechanical microvalve for tissue engineering
AU - Lee, W.
AU - Lee, V. K.
AU - Polio, S.
AU - Fischer, K.
AU - Lee, J. H.
AU - Park, J. K.
AU - Yoo, S. S.
PY - 2009
Y1 - 2009
N2 - In this study, we report a newly developed three-dimensional (3D) biological printer using non-contact, electromechanical microvalves with a nozzle diameter of 150 μm. To control and utilize this printer for life science applications, we developed an easy-to-use control software with a graphic user interface (GUI). First, using the printer, we tested the viability of dispensed mammalian cells after printing, and there was no significant difference in viability between dispensed cells and conventionally plated cells. Next, we constructed a 3D hydrogel scaffold by printing collagen hydrogel precursor layer-by-layer with linear patterns of gelatin inside. Using the same scheme, neurons were printed and patterned in multi-layered collagen scaffold. The on-demand capability to print cells and hydrogels in multi-layered hydrogel scaffold offers flexibility in generating artificial 3D tissue composites.
AB - In this study, we report a newly developed three-dimensional (3D) biological printer using non-contact, electromechanical microvalves with a nozzle diameter of 150 μm. To control and utilize this printer for life science applications, we developed an easy-to-use control software with a graphic user interface (GUI). First, using the printer, we tested the viability of dispensed mammalian cells after printing, and there was no significant difference in viability between dispensed cells and conventionally plated cells. Next, we constructed a 3D hydrogel scaffold by printing collagen hydrogel precursor layer-by-layer with linear patterns of gelatin inside. Using the same scheme, neurons were printed and patterned in multi-layered collagen scaffold. The on-demand capability to print cells and hydrogels in multi-layered hydrogel scaffold offers flexibility in generating artificial 3D tissue composites.
KW - 3D freeform fabrication
KW - Cell printing
KW - Electromechanical microvalve
KW - Hydrogel scaffold
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=71449118323&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=71449118323&partnerID=8YFLogxK
U2 - 10.1109/SENSOR.2009.5285591
DO - 10.1109/SENSOR.2009.5285591
M3 - Conference contribution
AN - SCOPUS:71449118323
SN - 9781424441938
T3 - TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 2230
EP - 2233
BT - TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
T2 - TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Y2 - 21 June 2009 through 25 June 2009
ER -