Three dimensional co-culture of neuron and astrocyte in a micro-fluidic device

Young Hun Kim, Young Eun Kim, Suk Chung, Byungkyu Kim, Tae Song Kim, Ji Yoon Kang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we describe a microfluidic platform that enables three dimensional cell culture within defined microenvironments towards a study of axon-glia interaction. The microdevice can offer both 3D axon isolation from cell body and 3D spatial cell separation between neuron and astrocyte. It consists of large fluidic channels for media supply and small channels for collagen filling as a three dimensional ECM (extracellular matrix). Astrocytes were seeded in collagen gel through small fluidic channels and neuron cells were aggregated at an openings of small channel to large channels. This method separated neuronal soma from axon as well as segregatedneuron body from astrocyte. The preliminary study on the effect of astrocyte to the axon growth showed that axon growth was faster and richer in co-culture condition without growth factor. This 3D co-culture chip can provide a new method to observe the effect of astrocyte on the axon growth in three dimensional culture system.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages852-854
Number of pages3
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Keywords

  • 3D cell culture
  • Astrocyte
  • Co-culture
  • Microfluidic chip
  • Neuron

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Kim, Y. H., Kim, Y. E., Chung, S., Kim, B., Kim, T. S., & Kang, J. Y. (2011). Three dimensional co-culture of neuron and astrocyte in a micro-fluidic device. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 852-854). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).