TY - JOUR
T1 - Three-dimensional differential interference contrast microscopy using synthetic aperture imaging
AU - Kim, Moonseok
AU - Choi, Youngwoon
AU - Fang-Yen, Christopher
AU - Sung, Yongjin
AU - Kim, Kwanhyung
AU - Dasari, Ramachandra R.
AU - Feld, Michael S.
AU - Choi, Wonshik
N1 - Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (grant numbers 2011-0005018 and 2011-0016568), the National R&D Program for Cancer Control, the Ministry of Health & Welfare, the Republic of Korea (1120290), the IT Research and Development Program of MKE/KEIT (KI001810039169), the National Center for Research Resources of the National Institutes of Health (P41-RR02594-24), and the National Science Foundation (DBI-0754339).
PY - 2012/2
Y1 - 2012/2
N2 - We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.
AB - We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.
KW - image processing
KW - interferometry
KW - microscopy
KW - synthetic apertures
UR - http://www.scopus.com/inward/record.url?scp=84863233755&partnerID=8YFLogxK
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U2 - 10.1117/1.JBO.17.2.026003
DO - 10.1117/1.JBO.17.2.026003
M3 - Article
C2 - 22463035
AN - SCOPUS:84863233755
VL - 17
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 2
M1 - 026003
ER -