Three-dimensional differential interference contrast microscopy using synthetic aperture imaging

Moonseok Kim, Youngwoon Choi, Christopher Fang-Yen, Yongjin Sung, Kwanhyung Kim, Ramachandra R. Dasari, Michael S. Feld, Wonshik Choi

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26 Citations (Scopus)

Abstract

We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.

Original languageEnglish
Article number026003
JournalJournal of Biomedical Optics
Volume17
Issue number2
DOIs
Publication statusPublished - 2012 Feb 1

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Keywords

  • image processing
  • interferometry
  • microscopy
  • synthetic apertures

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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