Three-dimensional interferometric scattering microscopy via remote focusing technique

Il Buem Lee, Hyeon Min Moon, Jin Sung Park, Katerina Zambochova, Seok Cheol Hong, Minhaeng Cho

Research output: Contribution to journalArticlepeer-review

Abstract

Interferometric scattering (iSCAT) microscopy enables us to track nm-sized objects with high spatial and temporal resolutions and permits label-free imaging of biomolecules. Its superb sensitivity, however, comes at a cost by several downsides, such as slow three-dimensional imaging and limited vertical tracking. Here, we propose a new method, Remote Focusing-iSCAT (RF-iSCAT) microscopy, to visualize a volume specimen by imaging sections at different depths without translation of either the objective lens or sample stage. We demonstrate the principle of RF-iSCAT by determining the z-position of submicrometer beads by translating the reference mirror instead. RF-iSCAT features an unprecedentedly long range of vertical tracking and permits fast but vibration-free vertical scanning. We anticipate that RF-iSCAT would enhance the utility of iSCAT for dynamics study.

Original languageEnglish
Pages (from-to)2628-2631
Number of pages4
JournalOptics Letters
Volume45
Issue number9
DOIs
Publication statusPublished - 2020 May 1

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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