Three-dimensional structure of the cytoskeleton in Trichomonas vaginalis revealed new features

Kyung Eun Lee, Ji Hui Kim, Min Kyo Jung, Tatsuo Arii, Jae Sook Ryu, Sung Sik Han

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The flagellated protozoan Trichomonas vaginalis has been widely studied owing to its medical significance and unique structure. The complicated three-dimensional (3D) structure of the cellular components of T. vaginalis was reconstructed from serial sections to enable observation of the spatial features of the whole cell. Electron tomography was used to examine the detailed structure of the cellular organelles. Tomographic reconstruction showed the mastigont system and the parabasal filament of T. vaginalis in detail. The last thin filament (Pf3) was located close to the adjacent filament, and the two filaments appeared to be vertically parallel in the cross-sectional view. It is likely that Pf3 cannot be distinguished from the adjacent filament in 2D images obtained from transmission electron microscopy. Our 3D reconstruction of T. vaginalis revealed the presence of an additional striated fiber, and 3D reconstruction by electron tomography showed twisting of the split parabasal filament.

Original languageEnglish
Pages (from-to)305-313
Number of pages9
JournalJournal of Electron Microscopy
Volume58
Issue number5
DOIs
Publication statusPublished - 2009 Oct 1

Fingerprint

Trichomonas vaginalis
Cytoskeleton
Tomography
filaments
Electron Microscope Tomography
Electrons
Cellular Structures
Transmission electron microscopy
Fibers
tomography
Transmission Electron Microscopy
Organelles
organelles
Observation
twisting
electrons
transmission electron microscopy
fibers
cells

Keywords

  • 3D structure
  • Additional parabasal filament
  • Electron tomography
  • Flagellar beating
  • T. vaginalis
  • Twisting structure

ASJC Scopus subject areas

  • Instrumentation

Cite this

Three-dimensional structure of the cytoskeleton in Trichomonas vaginalis revealed new features. / Lee, Kyung Eun; Kim, Ji Hui; Jung, Min Kyo; Arii, Tatsuo; Ryu, Jae Sook; Han, Sung Sik.

In: Journal of Electron Microscopy, Vol. 58, No. 5, 01.10.2009, p. 305-313.

Research output: Contribution to journalArticle

Lee, Kyung Eun ; Kim, Ji Hui ; Jung, Min Kyo ; Arii, Tatsuo ; Ryu, Jae Sook ; Han, Sung Sik. / Three-dimensional structure of the cytoskeleton in Trichomonas vaginalis revealed new features. In: Journal of Electron Microscopy. 2009 ; Vol. 58, No. 5. pp. 305-313.
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