Three-dimensional tumor model on micro-concave arrayed wells for screening of anticancer drug-loaded nanoparticles

A. Kang, H. Seo, B. Chung, Sang Hoon Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We investigated the effect of anticancer drug-loaded polymeric nanoparticles on three-dimensional breast tumor spheroids. To build MCF7 tumor models, we used concave microwell arrays with 300, 500 and 700 μm in diameter. Doxorubicin-loaded nanoparticles were prepared using thermo-responsive poly(N-isopropylacrylamide) (PNIPAM)-co-acrylic acid (AA). The smallest tumor spheroids cultured in 300 μm microwells were largely disrupted, resulting in a decrease in spheroid diameters upon culturing with doxorubicin-loaded nanoparticles for 96 hours. We found less size difference of largest tumor spheroids and cell viability assay also showed that largest tumor spheroids were highly resistant to the anticancer drug. Therefore, this size-controllable 3D breast tumor model could be a potentially powerful tool for anticancer drug screening applications.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages2576-2577
Number of pages2
ISBN (Electronic)9780979806476
Publication statusPublished - 2014 Jan 1
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 2014 Oct 262014 Oct 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period14/10/2614/10/30

Keywords

  • Anticancer drug screening
  • Concave microwell array
  • Drug resistance
  • Three-dimensional tumor model
  • Uniform-sized tumor

ASJC Scopus subject areas

  • Control and Systems Engineering

Fingerprint Dive into the research topics of 'Three-dimensional tumor model on micro-concave arrayed wells for screening of anticancer drug-loaded nanoparticles'. Together they form a unique fingerprint.

Cite this