Three novel C1q domain containing proteins from the disk abalone Haliotis discus discus

Genomic organization and analysis of the transcriptional changes in response to bacterial pathogens

S. D N K Bathige, Navaneethaiyer Umasuthan, J. D H E Jayasinghe, G. I. Godahewa, Hae Chul Park, Jehee Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The globular C1q (gC1q) domain containing proteins, commonly referred as C1q domain containing (C1qDC) proteins, are an essential family of proteins involved in various innate immune responses. In this study, three novel C1qDC proteins were identified from the disk abalone (Haliotis discus discus) transcriptome database and designated as AbC1qDC1, AbC1qDC2, and AbC1qDC3. The cDNA sequences of AbC1qDC1, AbC1qDC2, and AbC1qDC3 consisted of 807, 1305, and 660 bp open reading frames (ORFs) encoding 269, 435, and 220 amino acids (aa), respectively. Putative signal peptides and the N-terminal gC1q domain were identified in all three AbC1qDC proteins. An additional predicted motif region, known as the coiled coil region (CCR), was identified next to the signal sequence of AbC1qDC2. The genomic organization of the AbC1qDCs was determined using a bacterial artificial chromosome (BAC) library. It was found that the CDS of AbC1qDC1 was distributed among three exons, while the CDSs of AbC1qDC2 and AbC1qDC3 were distributed between two exons. Sequence analysis indicated that the AbC1qDC proteins shared

Original languageEnglish
Pages (from-to)181-187
Number of pages7
JournalFish and Shellfish Immunology
Volume56
DOIs
Publication statusPublished - 2016 Sep 1

Fingerprint

Haliotis discus
abalone
Pathogens
genomics
pathogen
protein
pathogens
Proteins
proteins
Protein Sorting Signals
signal peptide
exons
Exons
bacterial artificial chromosomes
Chromosomes
immune response
transcriptome
peptide
open reading frames
analysis

Keywords

  • C1q domain containing (C1qDC) protein
  • Genomic structure
  • Haliotis discus discus
  • Immune response

ASJC Scopus subject areas

  • Aquatic Science
  • Environmental Chemistry

Cite this

Three novel C1q domain containing proteins from the disk abalone Haliotis discus discus : Genomic organization and analysis of the transcriptional changes in response to bacterial pathogens. / Bathige, S. D N K; Umasuthan, Navaneethaiyer; Jayasinghe, J. D H E; Godahewa, G. I.; Park, Hae Chul; Lee, Jehee.

In: Fish and Shellfish Immunology, Vol. 56, 01.09.2016, p. 181-187.

Research output: Contribution to journalArticle

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